[Histonet] cutting MMA cross section of mouse femur

[Dorothy Hu]

Dear Histonetters,

I normally use 85/15 ratio of MMA/dibutyl phthalate (DP) in my mouse tibia
and femur longitudinal sections. But for cross section of mouse femur. Do
you change the ratio of MMA/DP to make block more soft or hard? It is
difficulty to get good cross sections. Any expert especially from hard
tissue committee, please help. I'm very much appreciated.

Dorothy Hu
MGH endocrine