[Histonet] RE: EDTA decalcification solution follow up questions

[Martha Ward-Pathology]

Thank you to everyone who has responded.   They have a more specific question:


Currently they are using SurgiPath DeCal solution II.     They are being asked to use an EDTA decal solution that will provide the best preservation of RNA and DNA proteins.   What is everyone using for this purpose?

Thanks again for any information....


 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mward <@t> wakehealth.edu  
 
 





-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology
Sent: Monday, December 01, 2014 2:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] EDTA decalcification solution

I am asking this question for our Histology Lab.  They are being asked about using EDTA as a decal solution for bone and wondered if anyone else is using this?    Is this available as a ready to use?   Or do you have to make it up?   What vendors are you using and could you provide a procedure about how you use it....length of time in solution, etc.

Thanks in advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890 mward <@t> wakehealth.edu  
 
 






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