[Histonet] IHC Validation:

[Jb]

How do most people validate IHC?  I want to create a tissue microarray. I wanted to use an average of 6-8 positive tissues. 

Is it necessary to validate using negative tissues when there is always an internal negative control in all tissue sections. Now with new polymer detection systems there is not background, etc. 

Is IHC validation ultimately up to the discretion of the laboratory director?

Please advise. Thx- 

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