رد: [Histonet] RE: processing biopsies on VIP

[j.rowaihi]

Hello colleagues
I am using this machine since long time. 
P/V mostly is not the cause of tissue drying, 
Check the wax baths temperature if it's not over 2 degrees from the paraffin wax melting point. 
Make sure the temperature in Xylene steps is not over 37 C degree.
Check the purity of the ethanol, specially the absolute ethanol. 


Regards

Jamal Rowaihi
Anatomic Pathology Supervisor
Al Borg Medical Laboratories

*Sent from my cell phoner*

-------- الرسالة الأصلية --------
من: Ian R Bernard <ibernard <@t> uab.edu> 
التاريخ: ٢٠١٤/٠٨/٢٩  ٦:٥٢ ص  (GMT+04:00) 
إلى: "Algeo, Lacie A" <Lacie.Algeo <@t> providence.org>,histonet <@t> lists.utsouthwestern.edu 
الموضوع: [Histonet] RE: processing biopsies on VIP 
 
Check your protocol.  Heat  or temp is a factor and length of time in alcohol. We have P/V for the 10 % NBF and paraffin infiltration.
IB
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A
Sent: Thursday, August 28, 2014 4:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing biopsies on VIP

Hi there,
Does anyone use P/V on their biopsy runs on the Tissue-Tek VIP 5?  We're having really dry biopsies, and I think this could be why....
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.algeo <@t> providence.org<mailto:lacie.algeo <@t> providence.org>


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