[Histonet] RE: Slide remounting

[James Watson]

With slides this old you can usually spray the back of the slide with freeze spray and the coverslip will pop off.  Then soak in xylene and once any remaining permount is gone, re-coverslip.  We did this routinely at AFIP,  in the old days.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel    858-332-4647
Fax   858-812-1915
jwatson <@t> gnf.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)
Sent: Wednesday, August 27, 2014 8:13 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide remounting
Importance: High


Does anyone have a procedure that removes coverslips (w/crystallized permount present) ? The slides are 10+ years old.  And is there a process to liquify the crystallized permount and refill w/o removing the coverslip?  The mounted slides are 3 x 1's

Juan l. Bassett HT ASCP
Histotechnician Anatomical Div.
National Museum of Health and Medicine
2500 Linden Lane 
Silver Spring, MD 20910
Office 301-319-3359
Lab      301-319-3328
Mobile 240-505-7247

Juan.l.bassett.ctr <@t> mail.mil


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