[Histonet] whole mount immunofluorescence staining for mouse aorta

[Elizabeth Chlipala]

Mark

Not sure why they would want to do this for IF staining.  The only thing I can think of that is similar to this is in mouse models of atherosclerosis we have run "en Face" analysis of the mouse aorta.  The entire mouse aorta is dissected out, trimmed of fat and cut open via a specific procedure, the entire aorta is then stained with Sudan IV to demonstrate the plaques and then pinned on a tar plate, images are captured for image analysis.    It is also common in the studies to run aortic root analysis - we have done this on frozen sections with brightfield IHC.  We have experience in all of these aspects of tissue collection and preparation, some of the techniques may apply to what they are trying to do.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Tuesday, August 19, 2014 3:48 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] whole mount immunofluorescence staining for mouse aorta

A colleague of mine has asked if I have any experience wholemount immunofluorescence staining for mouse aorta.  I don't have any experience with this so thought I would ask the group for any suggestions/protocols/ tips etc.
Thanks 
Mark