[Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129,
Issue 18
gayle callis
gayle.callis <@t> bresnan.net
Wed Aug 13 09:42:02 CDT 2014
The best way to determine the final endpoint is radiography as it is the
most sensitive if you have a FAXITRON. If you have this xray machine or a
microCT, then you should use these for decalcification endpoint and not
waste time with weight loss/weight gain method or any other method.
When we do the weight loss/weight gain, we simply made the judgement call
that the weight gain means no calcium is present and no further testing is
required. It is considered bad practice to "prick" the bone as you may 1)
create needle tract artifact into critical areas you might want to see 2)
possibly dislodge a small pathological lesion from the bone 3) bending bone
can dislodge tumor from bone . Consequently, we never did mechanical
testing EVER! We would decalcify as many as 40 to 50 samples - a
collection of NBF fixed mouse knees, or tibias, or paws at one time in a
large 1 to 2 liters 10% to 15% formic acid. Having to weigh each and
every one of these small bones was tedious and time consuming. We found
that if we took representative samples of each kind of bone - 4 from
experimental group, and 4 from control group and use these bones as the wt
loss/wt gain samples, the remaining samples still decalcifying were so close
in size (and weight), we had excellent decalcification without damaging the
bone. One learns very quickly which murine leg bones decalcify faster than
other with paws taking much more time due to the tiny bones so tightly
packed together.
The original Mawhinney et al weight gain/weight loss publication tested the
last nitric acid change with a chemical test. Unfortunately, doing
chemical testing with EDTA is even more tedious.
Some people will actually let the bones sit a few hours longer in formic
acid decalcifiers to ensure the calcium is removed but you may over expose
and do acid hydrolysis the antigens, nuclei i.e. "overdecalcification" .
You can do this but I suggest testing the bones again so see if that weight
gain has increased or not. If not, then you may have left the bones in acid
too long. It doesn't take long to damage staining with acid exposure.
A chemical endpoint test is more accurate than weight loss/weight gain, but
you can't be doing a huge number of samples in one container of decalcifying
solution. This is probably the reason those authors did chemical test
after weight loss/weight gain but with single samples. I know of one
person who success doing chemical test for 4 to 5 bone slabs of same size
and approximate weight, decalcfified in an acid solution, and did the
chemical test from a single container of used, never stirred decalcifier for
those 4 or 5 large samples.
I will be happy to send the original publication to you via private email,
and the chemical test method.
Gayle M. Callis
HTL/HT/MT(ASCP)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
raghulj <@t> orchidpharma.com
Sent: Tuesday, August 12, 2014 10:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Weight Loss/Weight Gain Decal _Histonet Digest, Vol 129,
Issue 18
Dear all,
In the weight loss/gain method how would we fix the optimal endpoint to
stop the decal process based on weight gain? Will weighing omit the other
ways of judgement such as needle prick, physical examination. In our tox
experiments we deal with lot of femurs in one stretch and weighing method
looks interesting. Pl. comment.
J.Raghul
Senior Research Associate
Orchid Chemicals and Pharamaceuticals Limited Toxicology, Chennai,India.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: 12 August 2014 22:38
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 129, Issue 18
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"Re: Contents of Histonet digest..."
Today's Topics:
1. RE: On the lighter side... (Shirley A. Powell)
2. Bcl-2 (Heather Knight)
3. IHC Steam Strips: (Jb)
4. RE: Are Paraffin Blocks Biohazard (Michael LaFriniere)
5. RE: On the lighter side... (Mark Turner)
6. RE: On the lighter side... (Bernice Frederick)
7. Weight Loss/Weight Gain Decal (Wait, Trevor Jordan)
8. Re: Weight Loss/Weight Gain Decal (Jennifer MacDonald)
9. RE: Weight Loss/Weight Gain Decal (Wait, Trevor Jordan)
10. RE: Histonet Digest, Vol 129, Issue 17 (Mayer,Toysha N)
11. RE:On the lighter side... (Mayer,Toysha N)
12. Re: RE:On the lighter side... (Barry Rittman)
13. Re: Weight Loss/Weight Gain decalcification endpoint test
(gayle callis)
14. Consult for taking abs from RUO to IVD (Patsy Ruegg)
15. RE: On the lighter side... (Susan.Walzer <@t> HCAHealthcare.com)
16. RE: Cytospin validation (Jamal)
17. Re: On the lighter side... (Michael Ann Jones)
18. looking for TS replacement in Histology due to retirement
(Webb, Dorothy L)
----------------------------------------------------------------------
Message: 1
Date: Mon, 11 Aug 2014 13:03:38 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: RE: [Histonet] On the lighter side...
To: Ingles Claire <CIngles <@t> uwhealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE25BFB25FD10 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="iso-8859-1"
I agree. :)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Old histologists never die, they are just well fixed...
Claire
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Michael Ann Jones
[mjones <@t> metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...
25 years, ("what?s that in micron?s??) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com
On 8/11/14, 5:17 AM, "Edwards, Richard" <ree3 <@t> leicester.ac.uk> wrote:
>Sniffed my first formalin and saw first post-mortem November 1965.
>
_______________________________________________
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------------------------------
Message: 2
Date: Mon, 11 Aug 2014 13:18:57 -0400
From: Heather Knight <heather.l.knight3 <@t> gmail.com>
Subject: [Histonet] Bcl-2
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CABEU4_xVVjqyPu9o8=ONJA__mLNOXAcRmt6pK2oLcSAKp=nvAA <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hi everyone-
Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse
tissue? If so, can you share both the protocol and the antibody
information?
We have tried numerous antibodies over the years with very limited success.
Thank you for your help!!
Best,
Heather Knight
------------------------------
Message: 3
Date: Mon, 11 Aug 2014 10:18:53 -0700
From: Jb <craigak12 <@t> gmail.com>
Subject: [Histonet] IHC Steam Strips:
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <375F4EF9-47B6-46CD-B5B1-B511309EF4AA <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii
Does anyone use IHC steam strips in their decloaking chamber? The question
is do you run a new steam strip each run and log each individual run w/it's
own steam strip?
Thank you,
Sent from my iPhone
------------------------------
Message: 4
Date: Mon, 11 Aug 2014 17:43:34 +0000
From: Michael LaFriniere <Michael.LaFriniere <@t> ccplab.com>
Subject: RE: [Histonet] Are Paraffin Blocks Biohazard
To: Dawn Bugge <drbugge <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4A2A16B9707CE04E9CB6C82DC18C1D296A0A37 <@t> AHCMSASEXCH00.my.ahc.local>
Content-Type: text/plain; charset="us-ascii"
Dawn,
The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to
survive formalin fixation and routine processing protocols, the CDC web site
has additional information, In my laboratories I put all blocks in hazardous
waste for incineration disposal. It is not that costly just to be on the
safe side.
Michael R. LaFriniere, HT (ASCP)
Executive Director
Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A * Silver Spring, MD 20904
P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844
michael.lafriniere <@t> CCPLab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dawn Bugge
Sent: Friday, August 08, 2014 2:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Are Paraffin Blocks Biohazard
Hello Histo World!
Our pathologist for our private GI lab would like me to find out if anyone
has done a study to determine if the paraffin blocks, once they have been
processed, are considered biohazard. I have searched high and low and can
find many people stating that the blocks are not bioharzard, with the
exception of neurological tissue, but they don't state how they know this.
He would like me to reference an actual study to prove that someone has
actually looked into this.
Any one know of something like this? I know common sense would say that
once the tissues have been in formalin for hours, than on the processor for
hours that the tissue would be non biohazard and completely safe.
Thanks for your help :)
--
Dawn R Bugge HT(ASCP), Lab Manager
Seattle Histology
Dawns Usborne Books Website <http://x3128.myubam.com/>
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Mon, 11 Aug 2014 17:49:13 +0000
From: Mark Turner <mturner <@t> CSILaboratories.com>
Subject: RE: [Histonet] On the lighter side...
To: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>, Ingles Claire
<CIngles <@t> uwhealth.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<643626B74DE2814D8537057F40E1A10B0CAD409D <@t> CSI-MX-NODEA.CSI-LABS.local>
Content-Type: text/plain; charset="iso-8859-1"
Saves on embalming costs....
Mark Turner, Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shirley A.
Powell
Sent: Monday, August 11, 2014 1:04 PM
To: Ingles Claire; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
I agree. :)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Old histologists never die, they are just well fixed...
Claire
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Michael Ann Jones
[mjones <@t> metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...
25 years, ("what?s that in micron?s??) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com
On 8/11/14, 5:17 AM, "Edwards, Richard" <ree3 <@t> leicester.ac.uk> wrote:
>Sniffed my first formalin and saw first post-mortem November 1965.
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 11 Aug 2014 17:53:31 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] On the lighter side...
To: Mark Turner <mturner <@t> CSILaboratories.com>, "Shirley A. Powell"
<POWELL_SA <@t> mercer.edu>, Ingles Claire <CIngles <@t> uwhealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<62C639732D3F274DACED033EBDF6ADAF2F0F3167 <@t> evcspmbx2.ads.northwestern.edu>
Content-Type: text/plain; charset="iso-8859-1"
Or we'll live forever.....
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Turner
Sent: Monday, August 11, 2014 12:49 PM
To: Shirley A. Powell; Ingles Claire; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Saves on embalming costs....
Mark Turner, Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shirley A.
Powell
Sent: Monday, August 11, 2014 1:04 PM
To: Ingles Claire; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
I agree. :)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Old histologists never die, they are just well fixed...
Claire
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Michael Ann Jones
[mjones <@t> metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...
25 years, ("what?s that in micron?s??) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com
On 8/11/14, 5:17 AM, "Edwards, Richard" <ree3 <@t> leicester.ac.uk> wrote:
>Sniffed my first formalin and saw first post-mortem November 1965.
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Mon, 11 Aug 2014 19:26:57 +0000
From: "Wait, Trevor Jordan" <WaitT <@t> livemail.uthscsa.edu>
Subject: [Histonet] Weight Loss/Weight Gain Decal
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1407785218488.59806 <@t> livemail.uthscsa.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hello all! I'm currently doing some decalcification and was curious if
anyone had some particular advice about the weight loss/weight gain method.
I understand that when the decalcification process is complete, the tissue
block will begin to increase in weight. However, I'm confused when I should
record the weight for the block once they have been taken out of the EDTA
solution. You see, for the times I weighed the blocks before... the weights
were a little skewed because there were differing amounts of solution on the
blocks while they were sitting on the balance. I just want to standardize my
protocol a little more so that I can be sure the block is actually gaining
weight due to the calcium loss rather than just extra solution sitting on
the outside of the block. Would letting the blocks sit out of solution for
about 30 minutes before being weighed help with the matter? I know that the
blocks take on water once they are completely decalcified so I'm not sure
how much this will affect that.
Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD
Candidate Abilene Christian University Class of 2013 Graduate B.S.
Biochemistry
------------------------------
Message: 8
Date: Mon, 11 Aug 2014 12:33:51 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Weight Loss/Weight Gain Decal
To: "Wait, Trevor Jordan" <WaitT <@t> livemail.uthscsa.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF5E608C00.D3ABF733-ON88257D31.006B4264-88257D31.006B619D <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
I believe this was originally from Patsy Ruegg
Decalcification End Point: Weight Loss, Weight Gain
1. Blot sample to remove excess fixative
2. Weigh bone in mg, record as beginning weight
3. Next day, rinse bone, blot and weigh bone daily, record weight.
Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh
decalcifying solution.
4. When bone begins to GAIN weight, remove from decalcifying
solution, rinse and process.
YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY
Once calcium is totally removed (bone loses weight as this happens), water
replaces the calcium and weight begins to go up. This is the point at which
calcium should be totally gone. The original method used a chemical test at
the end to insure no calcium was in the decalcifying solution. If you do
this, you cannot stir the solution during decalcification. Be sure to
suspend bone in the solution to insure all sides of bone are in contact with
decalcification solution.
From: "Wait, Trevor Jordan" <WaitT <@t> livemail.uthscsa.edu>
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Date: 08/11/2014 12:29 PM
Subject: [Histonet] Weight Loss/Weight Gain Decal
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
Hello all! I'm currently doing some decalcification and was curious if
anyone had some particular advice about the weight loss/weight gain method.
I understand that when the decalcification process is complete, the tissue
block will begin to increase in weight. However, I'm confused when I should
record the weight for the block once they have been taken out of the EDTA
solution. You see, for the times I weighed the blocks before... the weights
were a little skewed because there were differing amounts of solution on the
blocks while they were sitting on the balance.
I just want to standardize my protocol a little more so that I can be sure
the block is actually gaining weight due to the calcium loss rather than
just extra solution sitting on the outside of the block. Would letting the
blocks sit out of solution for about 30 minutes before being weighed help
with the matter? I know that the blocks take on water once they are
completely decalcified so I'm not sure how much this will affect that.
Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD
Candidate Abilene Christian University Class of 2013 Graduate B.S.
Biochemistry _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 9
Date: Mon, 11 Aug 2014 19:35:29 +0000
From: "Wait, Trevor Jordan" <WaitT <@t> livemail.uthscsa.edu>
Subject: RE: [Histonet] Weight Loss/Weight Gain Decal
To: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>,
"histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
Message-ID: <1407785730771.24250 <@t> livemail.uthscsa.edu>
Content-Type: text/plain; charset="iso-8859-1"
Ha Wow...that's almost too easy. Thank you for this!
Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD
Candidate Abilene Christian University Class of 2013 Graduate B.S.
Biochemistry ________________________________
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Sent: Monday, August 11, 2014 2:33 PM
To: Wait, Trevor Jordan
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Weight Loss/Weight Gain Decal
I believe this was originally from Patsy Ruegg
Decalcification End Point: Weight Loss, Weight Gain
1. Blot sample to remove excess fixative
2. Weigh bone in mg, record as beginning weight
3. Next day, rinse bone, blot and weigh bone daily, record weight.
Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh
decalcifying solution.
4. When bone begins to GAIN weight, remove from decalcifying
solution, rinse and process.
YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY
Once calcium is totally removed (bone loses weight as this happens), water
replaces the calcium and weight begins to go up. This is the point at which
calcium should be totally gone. The original method used a chemical test at
the end to insure no calcium was in the decalcifying solution. If you do
this, you cannot stir the solution during decalcification. Be sure to
suspend bone in the solution to insure all sides of bone are in contact with
decalcification solution.
From: "Wait, Trevor Jordan" <WaitT <@t> livemail.uthscsa.edu>
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Date: 08/11/2014 12:29 PM
Subject: [Histonet] Weight Loss/Weight Gain Decal
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
________________________________
Hello all! I'm currently doing some decalcification and was curious if
anyone had some particular advice about the weight loss/weight gain method.
I understand that when the decalcification process is complete, the tissue
block will begin to increase in weight. However, I'm confused when I should
record the weight for the block once they have been taken out of the EDTA
solution. You see, for the times I weighed the blocks before... the weights
were a little skewed because there were differing amounts of solution on the
blocks while they were sitting on the balance. I just want to standardize my
protocol a little more so that I can be sure the block is actually gaining
weight due to the calcium loss rather than just extra solution sitting on
the outside of the block. Would letting the blocks sit out of solution for
about 30 minutes before being weighed help with the matter? I know that the
blocks take on water once they are completely decalcified so I'm not sure
how much this will affect that.
Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD
Candidate Abilene Christian University Class of 2013 Graduate B.S.
Biochemistry _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Mon, 11 Aug 2014 19:45:01 +0000
From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
Subject: [Histonet] RE: Histonet Digest, Vol 129, Issue 17
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<47E9B2C01DDDD94881EACD2DC44EBC881B4382A0 <@t> D1PWPEXMBX05.mdanderson.edu>
Content-Type: text/plain; charset="us-ascii"
Egads, I feel like a baby!
20 years registered, 3 yrs unregistered.
By the way, I did see a former colleague with her ASCP certificate in her
office, and the oldest sticker is from the year I was born.
Sincerely,
Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator
Program in Histotechnology School of Health Professions UT M.D. Anderson
Cancer Center
713.563-3481
Message: 2
Date: Sun, 10 Aug 2014 21:50:58 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] On the lighter side...
To: Jamal <j.rowaihi <@t> alborglaboratories.com>, 'Vincent Rivera'
<vrivera <@t> westderm.com>, 'Douglas Porter' <doug.porter <@t> caplab.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF0C7C6D <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
38 years and looking forward to retirement in a few years.
Linda A. Sebree
------------------------------
Message: 11
Date: Mon, 11 Aug 2014 19:52:19 +0000
From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
Subject: [Histonet] RE:On the lighter side...
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<47E9B2C01DDDD94881EACD2DC44EBC881B4382E6 <@t> D1PWPEXMBX05.mdanderson.edu>
Content-Type: text/plain; charset="us-ascii"
Tim,
Just saw your post about the 'marketable skill'. Funny those were my
mother's exact words while I was in college. She didn't care what I majored
in, as long as I got a marketable skill along the way.
The best advice I've ever gotten.
Thanks Maria!!! (my mother)
Sincerely,
Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator
Program in Histotechnology School of Health Professions UT M.D. Anderson
Cancer Center
713.563-3481
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken,
Timothy
Sent: 08 August 2014 19:26
To: 'Douglas Porter'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369,
1992. Electron Microscopy Technologist, #604, 1982.
Like most, I never heard of "histology" until I walked into a hospital lab
on my first day as an EM tech. I had seen slides made in college, but no
one ever mentioned it could be an actual profession. I was more taken with
the electron microscope, and there was (is) a 2-year program at the
community college in the town I grew up in (Delta College, Stockton, CA). So
AFTER getting a BA in Zoology, I went there to get a marketable skill. At
that time EM was still used for tumor dx, so when I started it was about
half tumor, half kidney. I was lucky enough to get involved in histology and
set up the IHC lab at the small community hospital I worked at (as an EM
tech) and so ended up phasing myself almost out of an EM job. The IHC took
over all the tumor dx from EM. Later I left EM altogether and did IHC
exclusively for 15 years. But, like most, I learned Histotechnology on the
job but was lucky enough to work for a pathologist who believed in
developing his techs - to the point of paying for meetings out of his own
pocket. Only now do I know how fortunate I was to work for someone like
that. Because of him we had developed a culture in the small histo lab (4
men!!) of learning. We studied together one night a week for the HT exam and
all passed (and the practical!). Again, that was a fortunate experience, not
very often seen in labs.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center San Francisco, CA
------------------------------
Message: 12
Date: Mon, 11 Aug 2014 15:45:05 -0500
From: Barry Rittman <barryrittman <@t> gmail.com>
Subject: Re: [Histonet] RE:On the lighter side...
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CA+0tsF1QrppFYE2xSq+kt-8KX_fGd38Fvr657fYrZbY-4es2Qg <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Well fixed in the histological sense I hope.
Barry
On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N <TNMayer <@t> mdanderson.org>
wrote:
> Tim,
>
> Just saw your post about the 'marketable skill'. Funny those were my
> mother's exact words while I was in college. She didn't care what I
> majored in, as long as I got a marketable skill along the way.
> The best advice I've ever gotten.
> Thanks Maria!!! (my mother)
>
> Sincerely,
>
> Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education
> Coordinator Program in Histotechnology School of Health Professions UT
> M.D. Anderson Cancer Center
> 713.563-3481
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
> Sent: 08 August 2014 19:26
> To: 'Douglas Porter'; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] On the lighter side...
>
> Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369,
> 1992. Electron Microscopy Technologist, #604, 1982.
>
> Like most, I never heard of "histology" until I walked into a hospital lab
> on my first day as an EM tech. I had seen slides made in college, but no
> one ever mentioned it could be an actual profession. I was more taken with
> the electron microscope, and there was (is) a 2-year program at the
> community college in the town I grew up in (Delta College, Stockton, CA).
> So AFTER getting a BA in Zoology, I went there to get a marketable skill.
> At that time EM was still used for tumor dx, so when I started it was
about
> half tumor, half kidney. I was lucky enough to get involved in histology
> and set up the IHC lab at the small community hospital I worked at (as an
> EM tech) and so ended up phasing myself almost out of an EM job. The IHC
> took over all the tumor dx from EM. Later I left EM altogether and did IHC
> exclusively for 15 years. But, like most, I learned Histotechnology on the
> job but was lucky enough to work for a pathologist who believed in
> developing his techs - to the point of paying for meetings out of his own
> pocket. Only now do I know how fortunate I was to work for someone like
> that. Because of him we had developed a culture in the small histo lab (4
> men!!) of learning. We studied together one night a week for the HT exam
> and all passed (and the practical!). Again, that was a fortunate
> experience, not very often seen in labs.
>
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special
> Studies UC San Francisco Medical Center San Francisco, CA
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 13
Date: Mon, 11 Aug 2014 15:35:09 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Re: Weight Loss/Weight Gain decalcification
endpoint test
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601cfb5ac$22447d70$66cd7850$@bresnan.net>
Content-Type: text/plain; charset="us-ascii"
Trevor and Jennifer,
This method originally came from Mawhinney et al. Control of rapid nitric
acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy
Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and
Histochemistry. Mawhinney acutally did a chemical test on final acid change
to see that calcium was not present, but we never had to do that. If you
end up with a bit of residual calcium in block, I would surface decalcify at
microtomy. I used it for years when we downsized and gave away our
FAXITRON. Radiography is still the most accurate, and if you had either a
micro CT or digital FAXITRON available, it would be a better test. I
used Cathy's method and will be happy to send the original publication for
anyone's files/future referencing. A chemical test for EDTA is a pain to
do if a FAXITRON is not available.
I have put the full method below, with a bit more detail.
WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST
This is a method of choice for EDTA decalcification although it was
originally used by Mawhinney et al for testing nitric acid decalcification.
Many samples can be decalcified together in one container, i.e. 25 mouse
femurs in 1 liter of 10% formic acid. If all the samples are the same
size, i.e. mouse femurs, tibia, paws, choose several as representative
samples and test only those to save time. Always suspend bones in the
decalcifier.
Requires a balance that reads in milligrams to 3 places for greatest
accuracy. Specimen must be blotted free of fluid for accurate weighing each
time you weigh the sample. We suspend bones in nylon specimen bags for easy
removal to weigh. Bags can be marked with pencil too.
Technique:
1. Rinse NBF off bone, blot with paper towel, WEIGH BONE, RECORD BEGINNING
WEIGHT. Suspend bone in acid or EDTA decalcifier. During acid
decalcification CO2 bubbles are given off, so stir during decalcification to
release bubbles or small samples will float. EDTA does not create CO2
bubbles, only acids. Large bones can be started at end of day in acid
decalcifier and sit overnight with testing the next morning.
2. After 4 to 5 hours in acid or overnight in EDTA, remove bone, rinse with
water, BLOT, weigh. RECORD WEIGHT. If bone shows loss of weight, change
acid decalcifier to refresh acid. Return samples to resume decalcification,
and repeat as many times as necessary. EDTA should be changed but not as
often as acid. Always use a large volume of decalcifier i.e. 20:1 or more.
Remove bone from specimen bag, and place in weighing boat to protect balance
from acids/EDTA.
3. When bone begins to GAIN WEIGHT, the bone is decalcified. Once calcium
is removed, water is taken on and the weight increases. This water does not
affect the bone.
4. Rinse bone with running tap water for an hour or longer to remove these
decalcifiers. Either store in 70% alcohol or process. Store endpoint
tested decalcified bones in 70% alcohol while waiting for other samples to
finish decalcifying and mass processing run.
Reminders: For EDTA, one can suspend bones and check every day for accuracy
but bones can be left in the EDTA over a weekend or several days without
damage as long as the bones were well fixed. Acid decalcified bones cannot
be left over a weekend, remove from acid, put in NBF to stop
decalcification. Bones should be endpoint tested before stopping
decalcification so you can resume decalcification on the next working day.
Rinse off NBF briefly before resuming decalcification. Do not overexpose
bones to acids or you will damage antigens and nuclear staining.
Enjoy the method, as it truly is fast and easy.
Gayle M. Callis
HTL/HT/MT(ASCP)
____________________________________________________________________________
_____
Ha Wow...that's almost too easy. Thank you for this!
Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S. Biochemistry
________________________________
From: Jennifer MacDonald <
<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> JMacDonald <@t>
mtsac.edu>
Sent: Monday, August 11, 2014 2:33 PM
To: Wait, Trevor Jordan
Cc: <http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet
<@t> lists.utsouthwestern.edu;
<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet-bounces
<@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Weight Loss/Weight Gain Decal
I believe this was originally from Patsy Ruegg
Decalcification End Point: Weight Loss, Weight Gain
1. Blot sample to remove excess fixative
2. Weigh bone in mg, record as beginning weight
3. Next day, rinse bone, blot and weigh bone daily, record weight.
Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh
decalcifying solution.
4. When bone begins to GAIN weight, remove from decalcifying
solution, rinse and process.
YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY
Once calcium is totally removed (bone loses weight as this happens), water
replaces the calcium and weight begins to go up. This is the point at which
calcium should be totally gone. The original method used a chemical test at
the end to insure no calcium was in the decalcifying solution. If you do
this, you cannot stir the solution during decalcification. Be sure to
suspend bone in the solution to insure all sides of bone are in contact with
decalcification solution.
From: "Wait, Trevor Jordan" <
<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> WaitT <@t>
livemail.uthscsa.edu>
To: " <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
histonet <@t> lists.utsouthwestern.edu" <
<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> histonet <@t>
lists.utsouthwestern.edu>
Date: 08/11/2014 12:29 PM
Subject: [Histonet] Weight Loss/Weight Gain Decal
Sent by: <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
histonet-bounces <@t> lists.utsouthwestern.edu
________________________________
Hello all! I'm currently doing some decalcification and was curious if
anyone had some particular advice about the weight loss/weight gain method.
I understand that when the decalcification process is complete, the tissue
block will begin to increase in weight. However, I'm confused when I should
record the weight for the block once they have been taken out of the EDTA
solution. You see, for the times I weighed the blocks before... the weights
were a little skewed because there were differing amounts of solution on the
blocks while they were sitting on the balance. I just want to standardize my
protocol a little more so that I can be sure the block is actually gaining
weight due to the calcium loss rather than just extra solution sitting on
the outside of the block. Would letting the blocks sit out of solution for
about 30 minutes before being weighed help with the matter? I know that the
blocks take on water once they are completely decalcified so I'm not sure
how much this will affect that.
Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S. Biochemistry
_______________________________________________
Histonet mailing list
<http://lists.utsouthwestern.edu/mailman/listinfo/histonet> Histonet <@t>
lists.utsouthwestern.edu
<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Mon, 11 Aug 2014 17:04:32 -0600
From: Patsy Ruegg <pruegghm <@t> hotmail.com>
Subject: [Histonet] Consult for taking abs from RUO to IVD
To: "Histonet <@t> Lists. Edu" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY178-W34A801440A69FA187C6061D8ED0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Colleagues,
I am consulting with an antibody vendor who needs a consultant to help them
take their abs from RUO to IVD. If you know anyone who does this type of
work you could recommend would you please have them contact me directly and
I will pass their contact info on.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com
pruegg <@t> ihctech.net
------------------------------
Message: 15
Date: Tue, 12 Aug 2014 01:58:14 -0500
From: <Susan.Walzer <@t> HCAHealthcare.com>
Subject: RE: [Histonet] On the lighter side...
To: <CIngles <@t> uwhealth.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4BF03F5404EBDE409AF9232DA74B9DED2FBFFC425D <@t> FWDCWPMSGCMS09.hca.corpad.net>
Content-Type: text/plain; charset="iso-8859-1"
Also heard" Histotechs are good embed!!!" LOL
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...
Old histologists never die, they are just well fixed...
Claire
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Michael Ann Jones
[mjones <@t> metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...
25 years, ("what?s that in micron?s??) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com
On 8/11/14, 5:17 AM, "Edwards, Richard" <ree3 <@t> leicester.ac.uk> wrote:
>Sniffed my first formalin and saw first post-mortem November 1965.
>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Tue, 12 Aug 2014 10:49:24 +0300
From: "Jamal" <j.rowaihi <@t> alborglaboratories.com>
Subject: [Histonet] RE: Cytospin validation
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<013e01cfb601$f0da61e0$d28f25a0$@rowaihi <@t> alborglaboratories.com>
Content-Type: text/plain; charset="us-ascii"
Good day colleagues
Ignoring my previous message what it means:
no one did validation for Cytospin !!
or no one want to share his information ??
or no one received my message !!??
Best Regards,
Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg
Medical Laboratories | Mobile +966 503629832|
<mailto:j.rowaihi <@t> alborglaboratories.com> j.rowaihi <@t> alborglaboratories.com
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA |
Phone: +966 12 670 0099 | Fax: +966 12 676 4984 |
<http://www.alborglaboratories.com/> www.alborglaboratories.com
From: Jamal [mailto:j.rowaihi <@t> alborglaboratories.com]
Sent: Monday, August 11, 2014 1:47 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: Cytospin validation
Hi
I need to do validation for my Shandon Cytospin 4, can anyone send me the
validation procedure and form.
Best Regards,
Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg
Medical Laboratories | Mobile +966 503629832|
<mailto:j.rowaihi <@t> alborglaboratories.com> j.rowaihi <@t> alborglaboratories.com
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA |
Phone: +966 12 670 0099 | Fax: +966 12 676 4984 |
<http://www.alborglaboratories.com/> www.alborglaboratories.com
------------------------------
Message: 17
Date: Tue, 12 Aug 2014 13:00:08 +0000
From: Michael Ann Jones <mjones <@t> metropath.com>
Subject: Re: [Histonet] On the lighter side...
To: "Susan.Walzer <@t> HCAHealthcare.com" <Susan.Walzer <@t> HCAHealthcare.com>,
"CIngles <@t> uwhealth.org" <CIngles <@t> uwhealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D00F6BE1.2712%mjones <@t> metropath.com>
Content-Type: text/plain; charset="euc-kr"
Ha ha!! Good one -
Michael Ann
On 8/12/14, 12:58 AM, "Susan.Walzer <@t> HCAHealthcare.com"
<Susan.Walzer <@t> HCAHealthcare.com> wrote:
>Also heard" Histotechs are good embed!!!" LOL
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
>Claire
>Sent: Monday, August 11, 2014 12:00 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] On the lighter side...
>
>
>Old histologists never die, they are just well fixed...
>Claire
>________________________________________
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Michael Ann
>Jones [mjones <@t> metropath.com]
>Sent: Monday, August 11, 2014 9:07 AM
>To: Edwards, Richard; histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] On the lighter side...
>
>25 years, ("what??s that in micron??s???) Bernice, you are too funny!!
>(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
>Histology Manager Metropath
>7444 W. Alaska Dr. #250
>Lakewood, CO 80226
>303.634.2511
>Mjones <@t> metropath.com
>
>
>
>
>On 8/11/14, 5:17 AM, "Edwards, Richard" <ree3 <@t> leicester.ac.uk> wrote:
>
>>Sniffed my first formalin and saw first post-mortem November 1965.
>>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Tue, 12 Aug 2014 11:18:12 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] looking for TS replacement in Histology due to
retirement
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<65365F35C0F2EF4D846EC3CA73E49C4302F6D319B813 <@t> HPEMX3.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
Histology Technical Specialist Position in St. Paul, MN
Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching
hospital serving Minnesota and western Wisconsin for more than 130 years.
Regions is a private, non-profit hospital providing outstanding care in
women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn,
emergency care, and more. Regions Hospital Lab is a state of the art.
Regions is a part of the HealthPartners Family of Care. Additional
information is available at regionshospital.com
Regions Hospital employees enjoy opportunities for personal and professional
growth available only at one of the top teaching hospitals in the Twin
Cities area. Our dedication to patient care and commitment to a healthy
workplace, and "Best Care, Best Experience", has allowed us to be recognized
by the Minnesota Hospital Association with the Best Minnesota Hospital
Workplace Award.
This position will oversee the technical component of a work team in the
histology laboratory including; implementation of methodologies, supply
ordering and inventory management, interpretation of laboratory results for
physicians and other hospital staff; performance of laboratory tests in the
section assigned; assisting in performance evaluations; assisting in
supervision of testing personnel; development and implementation of training
and competency programs for histotechnicians and histology students interns,
and to perform related duties as assigned.
Qualifications:
Graduation from an accredited college or university with an Associates (AA)
or bachelor's degree in medical technology, histology, biology or related
field; plus specialized training in laboratory science. A master's degree in
related field may substitute for the above educational and registration
requirements. Bachelor's degree preferred.
At least four (4) years of experience as a Histotechnician or
Histotechnologist or related field with at least two (2) years of experience
in the specialty occurring within the last four years.
How to Apply
Apply online at
www.regionshospital.com<https://careers.peopleclick.com/careerscp/client_hea
lthpartners/regions_external/jobDetails.do?functionName=getJobDetail&jobPost
Id=59124&localeCode=en-us>
Additional Information
We are an Equal Opportunity Employer and do not discriminate against
applicants due to race, ethnicity, gender, veteran status, or on the basis
of disability or any other federal, state or local protected class.
________________________________
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