[Histonet] Water/Bubbles

[Laurie Colbert]

Hi Julie,

We have the Gemini stainer and have noticed that water gets trapped in the top of the slide rack.  Make sure the level of your dehydrating alcohols after the eosin are high enough to cover the top of the rack, and then make sure your xylene level is high enough to get rid of all the alcohol.  Also, avoid shaking the excess xylene off of the rack when you take it off the stainer before coverslipping.

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Julie Bowman
Sent: Thursday, August 07, 2014 1:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Water/Bubbles

Hello all,

Recently we have been experiencing a problem when running H&E slides on our Gemini stainer (Thermo Shandon Varistain). At the end of the run, small water droplets or possibly air bubbles are seen on the slides after taking the slides out of xylene. If they are coverslipped, a white haze will be seen around the specimen after a few minutes. Any suggestions of what causes this and how to correct it? Thank you!
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