[Histonet] RE: Bubble problems!

[Horn, Hazel V]

Melissa we are having the same problem.  We use a different mounting medium.  I will try Barbara's advice and try adding a bit of xylene to the media.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org






-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Thursday, August 07, 2014 8:12 AM
To: Melissa Ng; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Bubble problems!

Try adding xylene to the mounting medium to thin it.  About 10 ml of xylene to 90 ml of mounting medium should do it.
Then, leave the bottle of mounting medium for about a day or two to allow any air that may have gotten mixed into to dissipate.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.tibbs <@t> accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508


________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu> on behalf of Melissa Ng <melissa.ng <@t> spdscientific.com>
Sent: Thursday, August 07, 2014 7:59 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bubble problems!

Dear Histonet,



I am having a major problem with bubbles in my mounting media. I am currently using the Leica coverslipper and Cellpath mounting media (Phalate free). Micro-bubbles are observed on the slides after sometime of coverslipping. In fact, when I checked the media, I found lots of bubbles inside the bottle too! There were no bubbles in the bottle prior to placing the mounting media with the machine (I checked!). Also, we do not see any bubbles in the tubes that lead to the probe!



Previously, we were using the Leica mounting media (yellowish liquid) and there were no bubble issues. I have checked with other labs using the Cellpath mounting media (they use Leica coverslipper too) and no one seems to be having the same problem as us!



The Cellpath mounting media is way more viscous compared to that of the Leica media.



Has anyone here experienced a similar issue, and how have you resolved it?



Appreciate all the help I can get!



Best regards,

Melissa
Application Specialist
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