[Histonet] IHC validation:

[Cartun, Richard]

Many labs have turned to tissue microarrays (TMAs) for validating antibodies for IHC testing as a matter of convenience and lower cost.  Ideally, the tissues used to make the TMA(s) should be fixed in the formalin that is used in your laboratory, as well as processed in your Histology laboratory under identical conditions as those used for patient specimens.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Sunday, August 03, 2014 2:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC validation:

Does anyone have experience validating antibodies using tissue microarrays?  Is this a preferred method? I was thinking of punching different tissues/cases, then embedding them in one block, and then validating the antibody. Please advise.

Thank you.

Sent from my iPhone
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