[Histonet] Re: Negative Controls

Campbell, Tasha M. tmcampbell <@t> fmh.org
Wed Apr 30 06:47:54 CDT 2014

So this has confused me more. So before you would run a negative control
for each block you were testing and you would use the negative mouse or
rabbit reagent.  Now you don't have to do that but you still need a
negative tissue control.  So what exactly does this mean?  Does it mean
for every antibody that you are running you need to have a negative
tissue control for it?  So instead of using the negative mouse serum you
would run a known negative tissue control with the antibody, say CD3 or
whatever it is?  So are most people doing a control slide with a
negative tissue and a positive tissue on it?

Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144 (w)
304-685-9307 (c)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Terri
Sent: Tuesday, April 29, 2014 1:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Controls

On Message 7 - Negative Controls
While it is true that if you run polymers, you no longer have to run a
negative reagent control, HOWEVER, you still must have a negative tissue
control, which to quote CAP: "must  show no staining of tissues known to
lack the antigen"
Any of the following can serve as a negative tissue control:
1. Multi tissue blocks.  These can provide simultaneous positive and
negative tissue controls and are considered "best practice"...
The type of negative tissue control used (i.e. separate sections,
internal controls, or multitissue blocks) must be specified in the
laboratory manual."
Thus sayeth CAP, the almighty.  Please see ANP.22570
Our lab has defined our negative controls as a piece of Uterus as the
negative tissue in a multitissue block as a negative tissue control for
most of our antibodies, though for a few that might be too reactive in
uterus, we use a piece of skin.
I hope this helps.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Message: 7
From: Beth Brinegar <bbrinegarhtl <@t> gmail.com>
Subject: [Histonet] Negative controls
Hello fellow histonetters,

What is are other labs doing to satisfy the ANP.22570 QC - Antibodies
"Appropriate negative controls are used."



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