[Histonet] Xylene substitute for processing GI biopsies

Joe W. Walker, Jr. joewalker <@t> rrmc.org
Sun Apr 20 18:36:05 CDT 2014

Are you required to revalidate all of your IHC antibodies once you switch to a xylene sub?

We had utilized a xylene free process in our Peloris, which utilized alcohol but we ran into issue with FISH for Her2 and some of our other predictive markers and abandoned the alcohol.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference
Rutland Regional Medical Center
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies

Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping.
If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols.
If you follow the instructions, you will have a totally xylene free lab.
René J.
On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt <loreli173 <@t> mac.com> wrote:

Hi there!
I have spent a lot of time reading the archives, but still need some specific and current input/advice.
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute.  I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience!  This is completely new (to me), and rather overwhelming.
It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated.
I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point.

Thanks, in advance, for your help!

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