[Histonet] Xylene substitute for processing GI biopsies

Fri Apr 18 11:46:24 CDT 2014

We use propar on the tissue processor for mouse and rat eyes (you need three stations of proper) for adequate processing.  Works great for us, we do not use it in staining but I know it will work for that also.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Friday, April 18, 2014 10:41 AM
To: 'Blazek, Linda'; Lori Gemeinhardt; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies

We have used Americlear for many, many years.  Processing and staining of everything (not just GI).  It's a d-limonene product so it does have an orangish smell.  I have always had VIPs and we have never had a problem.

Tom McNemar, HT(ASCP)
Histology Supervisor
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Friday, April 18, 2014 11:40 AM
To: Lori Gemeinhardt; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitute for processing GI biopsies

Lori,

We use Formula 83 from CBG for both processing and staining.  We have used this for years with no problem for our GI biopsies.  It wouldn't do any good to share my processing schedule with you though since we have a different processor.  If you want to talk or any further information feel free to contact me.  We also recycle our Formula 83.

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, April 18, 2014 11:15 AM
To: Lori Gemeinhardt; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene substitute for processing GI biopsies

Hi Lori:
Instead of xylene you can use isopropyl (also known as 2-propanol and propyl alcohol) and after sectioning you can use dishwasher soap before going directly to the staining procedure. After staining you need nothing but an oven to dry out the stained sections and go diractly to coverslipping.
If you want details on the above please go to: http://www.histosearch.com/rene.html and read my articles on the subject, including processing protocols.
If you follow the instructions, you will have a totally xylene free lab.
René J.
On Thursday, April 17, 2014 8:19 PM, Lori Gemeinhardt <loreli173 <@t> mac.com> wrote:

Hi there!
I have spent a lot of time reading the archives, but still need some specific and current input/advice.
I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute.  I need insight on protocols, brands, effects on staining..... The good, the bad, the ugly. I have only used xylene in my many years of experience!  This is completely new (to me), and rather overwhelming.
It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated.
I understand this has been discussed, at length in the past, and I apologize.... I am just hoping to zero in on the best case scenario as a starting point.

Thanks, in advance, for your help!
Lori

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