[Histonet] RE: GI Biopsies

[Tony Reilly]

Hi Tasha

Formalin buffer salts are soluble in water but not alcohol.  I had a shredding problem once before when the blocks did not have adequate time in 70% Alcohol following formalin.  The buffer salts precipitate inside the tissue and cause shredding at microtomy.  This may not be your problem but as you are using a microwave processor and processing very rapidly it may be worth investigating.

Regards
Tony


Tony Reilly B.App.Sc,  M.Sc
Chief Scientist
Anatomical Pathology
Pathology Queensland PAH
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tasha M. Campbell
Sent: Friday, 4 April 2014 9:58 PM
To: Tony Reilly; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] GI Biopsies

Does anyone have problems with their GI biopsies kind of shredding when cutting?  I microwave process them in the Shurwave microwave processor.
I have been doing this for over a year and only the past couple months I have noticed that almost every biopsy I cut has knife lines in it.  I have to change my blade constantly.  I was told by a TBS technician that if I ever noticed the tissue doing this, it meant that I did not have all the alcohol out of the tissue, but does that sound like it would cause the tissue to shred like that?  I don't use my reagents more than
2 times and I have even tried increasing the time and temp a little but no help.  I have been doing them for a year and did not have a problem and I haven't changed anything.  Any solutions or suggestions???
Thanks!

 

 

 

 

Tasha Campbell, B.S.,HTL(ASCP)

Frederick Gastroenterology Associates

310 W. 9th St.

Frederick, MD 21701

301-695-6800 ext. 144 (w)

304-685-9307 (c)

 

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