[Histonet] Fatty Mammary Gland Processing

Freeman, Lisa* Lisa.Freeman <@t> fda.hhs.gov
Fri Sep 27 14:22:25 CDT 2013

I would greatly appreciate any input you have on processing fatty rodent mammary glands.

Our current processing protocol for mammary gland/large animal is 16 hours long.
1 hour X 8  ETOH (graded from 70% to 100%)
1 hour 20 minutes X 3 Xylene
1 hour 20 minutes X 3 paraffin

The rodents are very large and are not processing adequately. We have reprocessed the samples with success.

Thank you,

Lisa Freeman, HT
Histology Supervisor
Toxicologic Pathology Associates
National Center for Toxicological Research
3900 NCTR RD
Jefferson, AR 72079
Phone: 870-543-7234
E-mail: Lisa.Freeman <@t> fda.hhs.gov

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