[Histonet] edge effect on IHC

[Jan Shivers]

Also make sure that the tissue never dries out on the trimming table before
it even gets to formalin.  That can also be a source of edge effect.

Jan Shivers
UMN


On Wed, Sep 25, 2013 at 7:37 AM, Tim Higgins <thigginsht <@t> msn.com> wrote:

> Hey Mary,
>
>
>
> I would suggest observing your
> stainer to ensure the proper drop zones are being performed and not just
> dropping closest to the label. I used Dako for years and loved them but
> you do
> have to make sure it dispenses properly from time to time. They use drop
> zones
> unlike Ventana which uses a vortex type of mixing.
>
>
>
> Good luck!
>
>
>
> Thanks,
>
>
>
> Timothy N. Higgins, HT (ASCP), QIHC
>
>
>
>
>
> > Date: Mon, 23 Sep 2013 14:00:57 -0700 (PDT)
> > From: Mary Benoit <mbplab <@t> yahoo.com>
> > Subject: [Histonet] edge effect on IHC
> > To: "histonet <@t> lists.utsouthwestern.edu"
> >       <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <1379970057.43816.YahooMailNeo <@t> web142505.mail.bf1.yahoo.com>
> > Content-Type: text/plain; charset=us-ascii
> >
> > I have been aware of some "edge effect" on an occasional slide, but
> lately we noticed the effect is showing up in the area of the slide that is
> farthest from the labels. We use DAKO Link Autostainer and their
> pretreatment baths for everything. One case in particular we stained a
> large melanoma met to colon that was well fixed and processed that stained
> only the edge, however when we sent block to Neogenomics reference lab,
> their slide stained evenly over the entire tumor area. We both used a
> melanoma cocktail. Not sure what is going on. Any suggestions?
> > Thank you
> > Mary F Benoit, MT(ASCP)
> > The Pathology Laboratory
> > 830 Bayou Pines Drive
> > Lake Charles, LA 70601
> > mbplab <@t> yahoo.com
>
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