[Histonet] p40 antibody

Gary Gracie ggracie <@t> stvincents.com.au
Wed Sep 18 19:20:51 CDT 2013

Dear Natalie,

I can't help you with PAX8, and for Ki67 we use a Ventana pre-dilute. I can recommend Biocare Medical polyclonal p40, cat#ACI3030B.

I use it diluted 1/100.


Gary Gracie
Senior Technical Officer
IHC Laboratory
Anatomical Pathology
St Vincents Hospital
Sydney, Australia

Hello Everyone,
                      I am currently looking to bring a few new antibodies in house; KI-67,P40, and PAX8. I have not worked with these antibodies yet and wanted to get some opinions from everyone. For P40 and PAX8; are you using a monoclonal or polyclonal? Also what company are you getting them from? My hospital deals mainly with Dako, Biocare, and Cellmarque.  We are a very small facility that does HIER with a waterbath, and then manual IHC pipetting with the old sequenza coverplate tiles. So usually we prefer concentrate for antibodies not pre-dilute because of the longer expiration times, and of course the cost. So I guess what I am asking is, what clone do you prefer. What company do you get it from? How much does it cost? And have you ever run it on a manual setup, if so how was it to work with?
Thanks for all the help in advance,
Natalie J. Nagy
Histology Supervisor
Holyoke Medical Center
nagy_natalie <@t> holyokehealth.com

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