[Histonet] ZINC formalin vs NBF for IHC
Dolores_Fischer <@t> baxter.com
Wed Sep 18 08:27:26 CDT 2013
Good morning everyone!
I would like opinions on the use of zinc formalin for IHC. Pros and cons?? A new pathologist would like us to use zinc formalin for working out a protocol for CD34 and/or CD31 in mouse. I have always worked up needed protocols in 10% NBF with no problems and feel using zinc formalin will only throw in a variable that we don't need to add. I also feel that NBF is the fixative of choice for most antibodies and procedures. (small survey what do you use?) Am I not being open minded enough? What zinc formalin is recommended for use in a VIP processor and for IHC? I have read that there may be precipitation problems with this fixative. Opinions are appreciated, I did try to search the archives but didn't find too much information on this subject. I am trying to build a case for NOT using zinc formalin. I believe our pathologist is trying to save us the antigen retrieval step in IHC. Opinions??
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