[Histonet] ZINC formalin vs NBF for IHC

Fischer, Dolores Dolores_Fischer <@t> baxter.com
Wed Sep 18 08:27:26 CDT 2013

Good morning everyone!

I would like opinions on the use of zinc formalin for IHC.  Pros and cons??  A new pathologist would like us to use zinc formalin for working out a protocol for CD34 and/or CD31 in mouse.   I have always worked up needed protocols in 10% NBF with no problems and feel using zinc formalin will only throw in a variable that we don't need to add.  I also feel that NBF is the fixative of choice for most antibodies and procedures.  (small survey what do you use?)  Am I not being open minded enough? What zinc formalin is recommended for use in a VIP processor and for IHC?   I have read that there may be precipitation problems with this fixative.  Opinions are appreciated, I did try to search the archives but didn't find too much information on this subject.  I am trying to build a case for NOT using zinc formalin.  I believe our pathologist is trying to save us the antigen retrieval step in IHC.  Opinions??

Thanks all,


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