[Histonet] Nail specimens

[Rene J Buesa]

Cindy:
Nails need special treatment:
1- fix the nails for 24 hours
2- place them in a 10% aq. sol. of either sodium hydroxide or potassium hydroxide until when you touch them they are flexible. Wash them in water before you touch them. Softening the keratin in the nail will take about 30-45 minutes.
3- wash them in running water until when you touch them your fingers do not have a "soapy" feeling.
4- process and embed as usual
5- use (+) charged slides for the sections
6- heat the sections for 5-10 nim. at 60ºC in an oven before staining.
If you want I can send you a more detailed procedure.
René J.


________________________________
From: Cindy <caspake <@t> yahoo.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Tuesday, September 17, 2013 9:58 AM
Subject: [Histonet] Nail specimens


Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please!

Thanks,
Cindy

Sent from my iPhone
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