[Histonet] Re: Histonet Digest, Vol 118, Issue 24
Madeleine Huey
madeleinehuey <@t> gmail.com
Thu Sep 12 12:34:57 CDT 2013
O..o.oop! PC problem.
Haley,
I have experiences with all the above equipment’s and here's my inf;
1) Leica H&E Autostainer + glass coverslipper - smaller size therefore use
lesser counter space
2) Lesser cost $$ for Leica HE Autostainer / coverslipper
3) Bond Max/Bond III could do IHC & ISH simultaneously in one Autostainer,
while DAKO IHC autostainer can do only IHC (no heating elements)
4) Bond can do DELAY start while you are sleeping & continuos flow as well,
while DAKO need removed slides after AR & run immediately (batches)
Madeleine Huey BS, HTL/QIHC (ASCP)
Supervisor - IHC & Histology (Pathology)
On Thu, Sep 12, 2013 at 10:18 AM, Madeleine Huey <madeleinehuey <@t> gmail.com>wrote:
> From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
> >
> Sent: Wednesday, September 11, 2013 4:46 PM
> Subject: [Histonet] Equipment selection
>
> Hi all,
>
> I am trying to decide on what equipment to purchase. I have picked the
> processor, microtomes, embedding centers and a few other pieces, but now I
> still need to decide on the H&E Stainer and the immunostainer. My options I
> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
> Max. Any advice on any of these instruments would be greatly appreciated. I
> am trying to finalize my decision pretty quickly. Thank you in advance for
> any input.
>
> Haley,
> I have experiences with all the above equipments and here's my inf;
> 1) Leica H&E autostainer with glass coverslipper - smaller size (use
> lesser counter space)
> cheaper (buy 2 equivalent to 2 Sakura Prisma + film coverslipper and
> smaller.
>
>
> On Thu, Sep 12, 2013 at 10:02 AM, <
> histonet-request <@t> lists.utsouthwestern.edu> wrote:
>
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>> Today's Topics:
>>
>> 1. RE: Equipment selection (Huggins, Haley - MRMC)
>> 2. Re: Equipment selection (Rene J Buesa)
>> 3. RE: Equipment selection (Huggins, Haley - MRMC)
>> 4. RE: Equipment selection (Marcum, Pamela A)
>> 5. Need your expertise (Webb, Dorothy L)
>> 6. RE: Histonet Digest, Vol 118, Issue 23 (Tim Higgins)
>>
>>
>> ----------------------------------------------------------------------
>>
>> Message: 1
>> Date: Thu, 12 Sep 2013 08:55:39 -0700
>> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
>> Subject: RE: [Histonet] Equipment selection
>> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
>> "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <
>> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C69 <@t> PHX-MSG-007-N2.chw.edu>
>> Content-Type: text/plain; charset="iso-8859-1"
>>
>> I actually know all the equipment. Well, I know 3 of the pieces really
>> well, the Leica Bond Max is new to me. I did go to another lab to see it as
>> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
>> not as much with the coverslipper part of the stainer/coverslipper. The big
>> issue I am having is if I want to go with the Bond Max, I technically need
>> to go with the Leica stainer/coverslipper because it is lumped into a big
>> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
>> with the Dako immunostainer. I am having trouble deciding. It is boiling
>> down to money, which sucks since I might be forced to go with something
>> that I might not be happy with in the future.
>>
>> Haley
>>
>> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
>> Sent: Thursday, September 12, 2013 5:32 AM
>> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Equipment selection
>>
>> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
>> getting popularity. Why don't you go to a lab where they are in use and
>> take a look?
>> René J.
>>
>> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:
>> Haley.Huggins <@t> DignityHealth.org>>
>> To: "histonet <@t> lists.utsouthwestern.edu<mailto:
>> histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu
>> <mailto:histonet <@t> lists.utsouthwestern.edu>>
>> Sent: Wednesday, September 11, 2013 4:46 PM
>> Subject: [Histonet] Equipment selection
>>
>> Hi all,
>>
>> I am trying to decide on what equipment to purchase. I have picked the
>> processor, microtomes, embedding centers and a few other pieces, but now I
>> still need to decide on the H&E Stainer and the immunostainer. My options I
>> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
>> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
>> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
>> Max. Any advice on any of these instruments would be greatly appreciated. I
>> am trying to finalize my decision pretty quickly. Thank you in advance for
>> any input.
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu<mailto:
>> Histonet <@t> lists.utsouthwestern.edu>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>> ------------------------------
>>
>> Message: 2
>> Date: Thu, 12 Sep 2013 09:07:56 -0700 (PDT)
>> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> Subject: Re: [Histonet] Equipment selection
>> To: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>,
>> "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <1379002076.53612.YahooMailNeo <@t> web163105.mail.bf1.yahoo.com>
>> Content-Type: text/plain; charset=iso-8859-1
>>
>> If buying the Bond means that you are forced to "swallow" the Leica
>> coverslipper go with Sakura and Dako.
>> If they are making that "package" is because the Bond sells well but they
>> are having to "swallow" the coverslipper. Price is important but in the
>> long run it is better to increase productivity and avoid problems. The
>> Sakura is much faster than the Leica coverslipper.
>> René J.
>>
>>
>> ________________________________
>> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
>> To: Rene J Buesa <rjbuesa <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Thursday, September 12, 2013 11:55 AM
>> Subject: RE: [Histonet] Equipment selection
>>
>>
>>
>> I actually know all the equipment. Well, I know 3 of the pieces really
>> well, the Leica Bond Max is new to me. I did go to another lab to see it as
>> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
>> not as much with the coverslipper part of the stainer/coverslipper. The big
>> issue I am having is if I want to go with the Bond Max, I technically need
>> to go with the Leica stainer/coverslipper because it is lumped into a big
>> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
>> with the Dako immunostainer. I am having trouble deciding. It is boiling
>> down to money, which sucks since I might be forced to go with something
>> that I might not be happy with in the future.
>>
>> Haley
>>
>> From:Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
>> Sent: Thursday, September 12, 2013 5:32 AM
>> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Equipment selection
>>
>> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
>> getting popularity. Why don't you go to a lab where they are in use and
>> take a look?
>> RenéJ.
>>
>> From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
>> To: "histonet <@t> lists.utsouthwestern.edu" <
>> histonet <@t> lists.utsouthwestern.edu>
>> Sent: Wednesday, September 11, 2013 4:46 PM
>> Subject: [Histonet] Equipment selection
>>
>> Hi all,
>>
>> I am trying to decide on what equipment to purchase. I have picked the
>> processor, microtomes, embedding centers and a few other pieces, but now I
>> still need to decide on the H&E Stainer and the immunostainer. My options I
>> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
>> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
>> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
>> Max. Any advice on any of these instruments would be greatly appreciated. I
>> am trying to finalize my decision pretty quickly. Thank you in advance for
>> any input.
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> ------------------------------
>>
>> Message: 3
>> Date: Thu, 12 Sep 2013 09:10:47 -0700
>> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
>> Subject: RE: [Histonet] Equipment selection
>> To: Rene J Buesa <rjbuesa <@t> yahoo.com>,
>> "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <
>> 4F36EC93A5737D4F8A2974E8FB8E260615F5698C6D <@t> PHX-MSG-007-N2.chw.edu>
>> Content-Type: text/plain; charset="iso-8859-1"
>>
>> Thanks, that is a good point.
>>
>> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
>> Sent: Thursday, September 12, 2013 9:08 AM
>> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Equipment selection
>>
>> If buying the Bond means that you are forced to "swallow" the Leica
>> coverslipper go with Sakura and Dako.
>> If they are making that "package" is because the Bond sells well but they
>> are having to "swallow" the coverslipper. Price is important but in the
>> long run it is better to increase productivity and avoid problems. The
>> Sakura is much faster than the Leica coverslipper.
>> René J.
>>
>> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:
>> Haley.Huggins <@t> DignityHealth.org>>
>> To: Rene J Buesa <rjbuesa <@t> yahoo.com<mailto:rjbuesa <@t> yahoo.com>>; "
>> histonet <@t> lists.utsouthwestern.edu<mailto:
>> histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu
>> <mailto:histonet <@t> lists.utsouthwestern.edu>>
>> Sent: Thursday, September 12, 2013 11:55 AM
>> Subject: RE: [Histonet] Equipment selection
>>
>> I actually know all the equipment. Well, I know 3 of the pieces really
>> well, the Leica Bond Max is new to me. I did go to another lab to see it as
>> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
>> not as much with the coverslipper part of the stainer/coverslipper. The big
>> issue I am having is if I want to go with the Bond Max, I technically need
>> to go with the Leica stainer/coverslipper because it is lumped into a big
>> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
>> with the Dako immunostainer. I am having trouble deciding. It is boiling
>> down to money, which sucks since I might be forced to go with something
>> that I might not be happy with in the future.
>>
>> Haley
>>
>> From: Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
>> Sent: Thursday, September 12, 2013 5:32 AM
>> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu<mailto:
>> histonet <@t> lists.utsouthwestern.edu>
>> Subject: Re: [Histonet] Equipment selection
>>
>> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
>> getting popularity. Why don't you go to a lab where they are in use and
>> take a look?
>> René J.
>>
>> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org<mailto:
>> Haley.Huggins <@t> DignityHealth.org>>
>> To: "histonet <@t> lists.utsouthwestern.edu<mailto:
>> histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu
>> <mailto:histonet <@t> lists.utsouthwestern.edu>>
>> Sent: Wednesday, September 11, 2013 4:46 PM
>> Subject: [Histonet] Equipment selection
>>
>> Hi all,
>>
>> I am trying to decide on what equipment to purchase. I have picked the
>> processor, microtomes, embedding centers and a few other pieces, but now I
>> still need to decide on the H&E Stainer and the immunostainer. My options I
>> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
>> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
>> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
>> Max. Any advice on any of these instruments would be greatly appreciated. I
>> am trying to finalize my decision pretty quickly. Thank you in advance for
>> any input.
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu<mailto:
>> Histonet <@t> lists.utsouthwestern.edu>
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> ------------------------------
>>
>> Message: 4
>> Date: Thu, 12 Sep 2013 16:30:13 +0000
>> From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
>> Subject: RE: [Histonet] Equipment selection
>> To: "'Rene J Buesa'" <rjbuesa <@t> yahoo.com>, "Huggins, Haley - MRMC"
>> <Haley.Huggins <@t> DignityHealth.org>, "
>> histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <41D3A1AF6FEF0643BDC89E0516A6EA32D3158B57 <@t> Mail2Node2.ad.uams.edu>
>> Content-Type: text/plain; charset="iso-8859-1"
>>
>> Actually the coverslipper from Leica is very good and can be linked to
>> the stainer. It is also a glass coverslipper not tape. It is always about
>> money and that may be the thing that decides no matter what any of prefers.
>>
>> -----Original Message-----
>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
>> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
>> Sent: Thursday, September 12, 2013 11:08 AM
>> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Equipment selection
>>
>> If buying the Bond means that you are forced to "swallow" the Leica
>> coverslipper go with Sakura and Dako.
>> If they are making that "package" is because the Bond sells well but they
>> are having to "swallow" the coverslipper. Price is important but in the
>> long run it is better to increase productivity and avoid problems. The
>> Sakura is much faster than the Leica coverslipper.
>> René J.
>>
>>
>> ________________________________
>> From: "Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
>> To: Rene J Buesa <rjbuesa <@t> yahoo.com>; "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Sent: Thursday, September 12, 2013 11:55 AM
>> Subject: RE: [Histonet] Equipment selection
>>
>>
>>
>> I actually know all the equipment. Well, I know 3 of the pieces really
>> well, the Leica Bond Max is new to me. I did go to another lab to see it as
>> well as the Leica stainer/coverslipper. I was impressed with the Bond Max,
>> not as much with the coverslipper part of the stainer/coverslipper. The big
>> issue I am having is if I want to go with the Bond Max, I technically need
>> to go with the Leica stainer/coverslipper because it is lumped into a big
>> package deal. If I want the Sakura Prisma stainer/film coverslipper, I go
>> with the Dako immunostainer. I am having trouble deciding. It is boiling
>> down to money, which sucks since I might be forced to go with something
>> that I might not be happy with in the future.
>>
>> Haley
>>
>> From:Rene J Buesa [mailto:rjbuesa <@t> yahoo.com]
>> Sent: Thursday, September 12, 2013 5:32 AM
>> To: Huggins, Haley - MRMC; histonet <@t> lists.utsouthwestern.edu
>> Subject: Re: [Histonet] Equipment selection
>>
>> I used the Sakura and the Dako, but it seems that the Leica Bond Max is
>> getting popularity. Why don't you go to a lab where they are in use and
>> take a look?
>> RenéJ.
>>
>> From:"Huggins, Haley - MRMC" <Haley.Huggins <@t> DignityHealth.org>
>> To: "histonet <@t> lists.utsouthwestern.edu" <
>> histonet <@t> lists.utsouthwestern.edu>
>> Sent: Wednesday, September 11, 2013 4:46 PM
>> Subject: [Histonet] Equipment selection
>>
>> Hi all,
>>
>> I am trying to decide on what equipment to purchase. I have picked the
>> processor, microtomes, embedding centers and a few other pieces, but now I
>> still need to decide on the H&E Stainer and the immunostainer. My options I
>> have in front of me are for the H&E stainer are Sakura Prisma stainer/film
>> coverslipper and the Leica ST5020-CV5030 stainer/glass coverslipper. For
>> the Immunostainer, I am looking at the Dako Autostainer and the Leica Bond
>> Max. Any advice on any of these instruments would be greatly appreciated. I
>> am trying to finalize my decision pretty quickly. Thank you in advance for
>> any input.
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> ----------------------------------------------------------------------
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>>
>>
>> ------------------------------
>>
>> Message: 5
>> Date: Thu, 12 Sep 2013 11:45:41 -0500
>> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
>> Subject: [Histonet] Need your expertise
>> To: "'histonet <@t> lists.utsouthwestern.edu'"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <
>> 65365F35C0F2EF4D846EC3CA73E49C4302ACEFF2C823 <@t> HPEMX3.HealthPartners.int>
>>
>> Content-Type: text/plain; charset="us-ascii"
>>
>> We will finally be going green by recycling formalin. I would appreciate
>> any advice or opinions on true recycling VS. a filtering type of system. As
>> we all know, experience with a system or product is the best way to gain
>> true knowledge!
>>
>> Also, I would appreciate knowing how others are handling extra levels of
>> prostate needle bx's. We currently place them on charged slides and keep
>> until the case is signed out, but it seems there should be a better way of
>> handling this due to storage of the slides not to mention the extra cost
>> involved! I know this is becoming more of a needed process with all of the
>> RNA/DNA testing being done on diagnosed cancers.
>>
>> As always, I do appreciate any and all comments, advice, or words of
>> wisdom!! Hope to see some of you in Providence late next week!
>>
>> Dorothy Webb, HT (ASCP)
>> Regions Histology Technical Specialist
>> 651-254-2962
>>
>>
>>
>> ________________________________
>> This e-mail and any files transmitted with it are confidential and are
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>>
>>
>> ------------------------------
>>
>> Message: 6
>> Date: Thu, 12 Sep 2013 11:55:43 -0500
>> From: Tim Higgins <thigginsht <@t> msn.com>
>> Subject: [Histonet] RE: Histonet Digest, Vol 118, Issue 23
>> To: "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID: <BAY179-W92030A0D22235B31AF8D97D83A0 <@t> phx.gbl>
>> Content-Type: text/plain; charset="iso-8859-1"
>>
>> Hey Martha,
>>
>> You have to forgive Rene J. she speaks sometimes and has nothing good to
>> say but keeps speaking. There is never a bad question, if you don't have a
>> good answer then keep it to yourself as I usually do.
>>
>> "Adequate forum". really? I guess no one on Histonet is allowed to share
>> their personal protocols, procedures, opinions, and experiences on here
>> anymore.
>>
>> Come on Rene J., next time shhhhhhhhh!!
>>
>> I should have just hit the "Delete" button and follow my own advise!
>>
>> Tim N. Higgins, HT (ASCP), QIHC
>>
>>
>> > Message: 7
>> > Date: Thu, 12 Sep 2013 06:22:34 -0700 (PDT)
>> > From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>> > Subject: Re: [Histonet] Minimum sample size for breast markers
>> > To: Martha Ward-Pathology <mward <@t> wakehealth.edu>,
>> > "histonet <@t> lists.utsouthwestern.edu"
>> > <histonet <@t> lists.utsouthwestern.edu>
>> > Message-ID:
>> > <1378992154.20733.YahooMailNeo <@t> web163104.mail.bf1.yahoo.com>
>> > Content-Type: text/plain; charset=iso-8859-1
>> >
>> > I do not think this is the adequate forum to ask this question because
>> you may get a wrong answer that could determine your decision.
>> > Your pathologist is the one who should decide about this issue.
>> > René J.
>> >
>> >
>> > ________________________________
>> > From: Martha Ward-Pathology <mward <@t> wakehealth.edu>
>> > To: "histonet <@t> lists.utsouthwestern.edu" <
>> histonet <@t> lists.utsouthwestern.edu>
>> > Sent: Thursday, September 12, 2013 9:06 AM
>> > Subject: [Histonet] Minimum sample size for breast markers
>> >
>> >
>> >
>> >
>> > Is there a minimum number of cells that should be present when testing
>> for ER, PR and Her2? I have looked on the CAP website but cannot see
>> anything about this. We are constantly getting these small biopsies or
>> FNAs for testing and I sometimes wonder what the lowest threshold is for
>> testing...20 tumor cells, 50 tumor cells???? Any information would be
>> appreciated. Thanks in advance for your help.
>> >
>> > Martha Ward, MT (ASCP) QIHC
>> > Manager
>> >
>> > Molecular Diagnostics Lab
>> > Medical Center Boulevard \ Winston-Salem, NC 27157
>> > p 336.716.2109 \ f 336.716.5890
>> > mward <@t> wakehealth.edu
>> >
>>
>>
>> ------------------------------
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> End of Histonet Digest, Vol 118, Issue 24
>> *****************************************
>>
>
>
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