[Histonet] unsubscribe

Silverman, Jeffrey JSilverman <@t> NSHS.edu
Mon Sep 9 14:08:23 CDT 2013



Jeffrey S. Silverman HT HTL QIHC (ASCP)
Operations Manager
Anatomic Pathology Specimen Processing,
Laboratory Safety Officer

Lenox Hill Hospital
12 Uris- Anatomic Pathology
100 East 77th Street
New York, New York 10075

office- 212 434 6673
cell-631 375 4505
email:  jsilverman <@t> nshs.edu






-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, September 09, 2013 1:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 118, Issue 10

Send Histonet mailing list submissions to
        histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. RE: Cell Block Preparation (Cartun, Richard)
   2. Re: Unsubscribe, Chapter 195 (Emily Sours)
   3. (no subject) (Martin, Erin)
   4. RE: (no subject) (Manfre, Philip)
   5. RE: (no subject) (Marcum, Pamela A)
   6. RELIA Hot Histology Job Alert Histology Supervisor        needed in
      North Florida. (Pam Barker)
   7. Re: Unsubscribe, Chapter 195 (Lee & Peggy Wenk)
   8. Re: Unsubscribe, Chapter 195 (nmhisto <@t> comcast.net)
   9. ICC on previously Wright stained smears (Susan Foreman)
  10. Re: ICC on previously Wright stained smears (Rene J Buesa)
  11. Re: Unsubscribe, Chapter 195 (dusko trajkovic)
  12. Re: (no subject) (Jennifer MacDonald)
  13. RE: FISH enumeration (Sue Hunter)


----------------------------------------------------------------------

Message: 1
Date: Mon, 9 Sep 2013 00:20:12 +0000
From: "Cartun, Richard" <Richard.Cartun <@t> hhchealth.org>
Subject: RE: [Histonet] Cell Block Preparation
To: Ann Specian <thisisann <@t> aol.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9215BD4B0BA1B44D962A71C758B68D2E018820D5 <@t> HHCEXCHMB05.hhcsystem.org>
Content-Type: text/plain; charset="us-ascii"

We borrowed a wonderful cell block procedure from Windham Community Memorial Hospital here in CT several years ago.  The specimen is collected fresh in sterile saline or RPMI and then centrifuged to concentrate the cells.  Plasma and then thrombin are added to form a clot.  The clot is then fixed in formalin and processed in our Histology Laboratory.  I will send the procedure to anyone who is interested.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Ann Specian [thisisann <@t> aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation

I am getting complaints in regard to "insufficient" cell blocks.  We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper.

Does anyone have a more current technique which renders better cellularity?

Also, do you know which renders a better cell block:  a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?

Thanks,
Ann
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.



------------------------------

Message: 2
Date: Mon, 9 Sep 2013 07:32:25 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CAP=XX1zBwxubqfSKbeX7oz4=AVMWNRDTA3+gZ4jYa9xWbUPEJw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Excellent idea!!

"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Sat, Sep 7, 2013 at 4:57 PM, Amos Brooks <amosbrooks <@t> gmail.com> wrote:

> Hi,
>      I do not disagree at all. in fact, I think it would make for an
> interesting management tool. If you have the nicest CV around, but a simple
> Histonet archive search for your name + "unsubscribe" shows that you can't
> follow simple instructions, it says something about your communication
> skills and raises interesting questions about weather you are a good
> employment candidate. One might also look for the various incarnations of
> misspelled attempts at unsubscribing such as "unscribe" and "unsiscribe".
>
> Just sayin'
> Amos
>
> On Fri, Sep 6, 2013 at 1:00 PM,
> <histonet-request <@t> lists.utsouthwestern.edu>wrote:
>
> > Message: 6
> > Date: Fri, 6 Sep 2013 01:56:37 +0000 (UTC)
> > From: nmhisto <@t> comcast.net
> > Subject: [Histonet] Unsubscribe, Chapter 195
> > To: HISTONET  <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >         <
> >
> 1200426006.1029812.1378432597832.JavaMail.root <@t> sz0075a.emeryville.ca.mail.comcast.net
> > >
> >
> > Content-Type: text/plain; charset=utf-8
> >
> > It is a concern that members of our??  technically-oriented career field
> > ?? have a difficult time understanding the method for unsubscribing to
> > Histonet.??  There is an ?? almost- daily posting to "unsubscribe", despite
> > the fact that this subject has been addressed literally hundreds of
> > times.??  When one "joins" Histonet, instructions are provided, should be
> > printed out for reference and used?? if the subscriber decides to leave
> the
> > group.??  We are required to be knowledgeable on all manner of technical
> > routines requiring detailed instructions and??  Histonet is no less clear
> in
> > the methods for joining and "un-joining".??  Use them, please.??  Fire
> away -
> > I'm retired and I can take the flak!??  I do miss my microtome, though...
> >
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 3
Date: Mon, 9 Sep 2013 13:28:29 +0000
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Subject: [Histonet] (no subject)
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Cc: "Naujokas, Agne" <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier,    Melissa"
        <Melissa.Meier <@t> ucsfmedctr.org>
Message-ID: <24B7B291CC88D04AB663958E77A1F59D18144C <@t> ex09.net.ucsf.edu>
Content-Type: text/plain; charset=iso-8859-1

Good morning all!

One of our fellows emailed me a question that she came across while studying for her boards:



"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.  If you receive this in error, please contact the sender and delete the material from any computer.


------------------------------

Message: 4
Date: Mon, 9 Sep 2013 09:42:48 -0400
From: "Manfre, Philip" <philip_manfre <@t> merck.com>
Subject: RE: [Histonet] (no subject)
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>, histonet
        <histonet <@t> lists.utsouthwestern.edu>
Cc: "Naujokas, Agne" <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier,    Melissa"
        <Melissa.Meier <@t> ucsfmedctr.org>
Message-ID:
        <558A4571351D0C42BD923F403F4198C4B2701AE678 <@t> USCTMXP51014.merck.com>
Content-Type: text/plain; charset="us-ascii"

It's a bad question.  Generally the "best" temperature is probably between 55 and 65, depending on the paraffin.  This would fall between the two latter answers.  Hmm....  What to do?

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre <@t> merck.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 9:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying for her boards:



"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.  If you receive this in error, please contact the sender and delete the material from any computer.
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.




------------------------------

Message: 5
Date: Mon, 9 Sep 2013 13:50:06 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: RE: [Histonet] (no subject)
To: "'Martin, Erin'" <Erin.Martin <@t> ucsf.edu>, histonet
        <histonet <@t> lists.utsouthwestern.edu>
Cc: "Naujokas, Agne" <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier,    Melissa"
        <Melissa.Meier <@t> ucsfmedctr.org>
Message-ID:
        <41D3A1AF6FEF0643BDC89E0516A6EA32D3157DDE <@t> Mail2Node2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"

The last answer will cover most paraffins used routinely and may cover microwave also.  Temperatures below 58C are often melt points however; they may not be a complete liquid or have slightly increased viscosity.

Pam Marcum
UAMS

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 8:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying for her boards:



"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

Confidentiality Notice
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material.  Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited.  If you receive this in error, please contact the sender and delete the material from any computer.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

----------------------------------------------------------------------
Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.



------------------------------

Message: 6
Date: Mon, 9 Sep 2013 10:03:12 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Hot Histology Job Alert Histology Supervisor
        needed in North Florida.
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003f01cead65$52b52b30$f81f8190$@earthlink.net>
Content-Type: text/plain;       charset="us-ascii"

Hi Histonetters!!
I hope you all had a great weekend!! And I hope your teams NCAA and NFL did
better than mine did!!  I wanted to take a minute and tell you about a
position I recently was engaged on that I am very excited about.
RELIA has been engaged to recruit for a histology supervisor for a private
pathology lab located in North Florida.  This is a busy full staffed private
full service pathology lab which processes all types of tissue and runs a
full menu of special stains and IHC.  The ideal candidate for my client will
have at least 5 years of supervisory experience in a histology lab. HT/HTL
and Florida Supervisor license or elig are required.  My client offers an
excellent salary, benefits and relocation assistance.  For more information
please contact me - Pam Barker toll free at 866-607-3542 until 5pm EST or
anytime on my cell phone at 407-353-5070 or via email at
relia1 <@t> earthlink.net to set up a time to talk.

If you know of someone who might be interested remember RELIA offers a
referral bonus.  If we place someone you refer you will earn a referral
bonus.
Have a great Monday!!!

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker

Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:     (407)353-5070
FAX:     (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com <http://www.facebook.com/PamBarkerRELIA> /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia







------------------------------

Message: 7
Date: Mon, 9 Sep 2013 10:47:55 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: "Manfre, Philip" <philip_manfre <@t> merck.com>, <nmhisto <@t> comcast.net>,
        "HISTONET" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A5C7F7079C9A4D71B577654C9383BD5E <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="UTF-8";
        reply-type=original

I'm going to wade in, not as someone who has posted numerous times on how to
unsubscribe, but as someone assessing it from a "risk assessment"
evaluation.

If there is a lab task that is consistently being done wrong, by many
different people, it is usually NOT the fault of the people. It is either a
training issue, or a process problem. So we either have to do a better job
training and re-training, or we need to change how the process/procedure is
being done.

With the Histonet email, since people are constantly joining, often for a
day or two, we can't really improve the "training" aspect. Yes, there are
instructions when we first join, to print off/save how to subscribe or
unsubscribe or change personal information, etc. But (be honest) how many of
us pay attention to these types of instructions when we sign up to be a
"member" of a credit card or a on-line department store or an on-line book
store or other email lists? Most people do not. So we know that this type of
"training" is not effective. But we really can't do a one-on-one type of
training session for each person who signs on to Histonet. Therefore,
improving the training is not the answer.

The answer lies in modifying the process. Look at the bottom of those emails
from credit cards or hotels or department stores that you have signed up
with. There is usually a line that says "If you no longer wish to receive
these emails, click on this link and follow the instructions".

Add to that, various email lists have various methods on how to unsubscribe,
which can involve a link, or putting the word unsubscribe in the subject, or
putting the word unsubscribe in the message.

Histonet has a link at the bottom, but no instructions. So it's not clear to
click on the link to unsubscribe, nor is there any mention whether one of
the other unsubscribing methods would work. I therefore believe the Histonet
unsubscribing procedure has a process problem, that could be easily fixed.

As for the fact that how to unsubscribe has been explained 5,391+ times in
the past does not help the person who signed up over the weekend, and as of
today, decided that Histonet is not what they need. This new person has not
seen the previous requests for help with unsubscribing, nor the answers on
how to do it. Again, this is a process problem.

Is there any way Histonet can get some clearer instructions at the bottom of
each email, on how to unsubscribe, either permanently or temporarily while
on vacation? Such as saying "To unsubscribe, click on the link below, and
follow the instructions at the bottom of the next webpage".

Let's not yell at the people trying to unsubscribe. Let's work on improving
the unsubscribing process, so we don't get these requests.

Peggy A. Wenk, HTL(ASCP)SLS

-----Original Message-----
From: Manfre, Philip
Sent: Friday, September 06, 2013 7:30 AM
To: nmhisto <@t> comcast.net ; HISTONET
Subject: RE: [Histonet] Unsubscribe, Chapter 195

Trained professionals should know by now that if you want to unsubscribe,
you must type in all caps - UNSUBSCRIBE


Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre <@t> merck.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
nmhisto <@t> comcast.net
Sent: Thursday, September 05, 2013 9:57 PM
To: HISTONET
Subject: [Histonet] Unsubscribe, Chapter 195

It is a concern that members of our  technically-oriented career field  have
a difficult time understanding the method for unsubscribing to Histonet.
There is an  almost- daily posting to "unsubscribe", despite the fact that
this subject has been addressed literally hundreds of times.  When one
"joins" Histonet, instructions are provided, should be printed out for
reference and used if the subscriber decides to leave the group.  We are
required to be knowledgeable on all manner of technical routines requiring
detailed instructions and  Histonet is no less clear in the methods for
joining and "un-joining".  Use them, please.  Fire away - I'm retired and I
can take the flak!  I do miss my microtome, though...
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.






_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 8
Date: Mon, 9 Sep 2013 14:58:09 +0000 (UTC)
From: nmhisto <@t> comcast.net
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>
Cc: HISTONET <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <670394625.1111582.1378738689536.JavaMail.root <@t> sz0075a.emeryville.ca.mail.comcast.net>

Content-Type: text/plain; charset=utf-8

I??was happy to read your thoughtful evaluation of the problem and, after having read through it, wonder why I hadn't thought of it before!?? Perhaps a little "tweaking" by the listserv management would make this process much more clear.?? The sign-on page of information about Histonet is rather lengthy (rightly so) but perhaps it's time for a little updating.?? Thank you, Peggy, for your thoughts and proposed solution.

----- Original Message -----
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
To: "Philip Manfre" <philip_manfre <@t> merck.com>, nmhisto <@t> comcast.net, "HISTONET" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, September 9, 2013 8:47:55 AM
Subject: Re: [Histonet] Unsubscribe, Chapter 195

I'm going to wade in, not as someone who has posted numerous times on how to
unsubscribe, but as someone assessing it from a "risk assessment"
evaluation.

If there is a lab task that is consistently being done wrong, by many
different people, it is usually NOT the fault of the people. It is either a
training issue, or a process problem. So we either have to do a better job
training and re-training, or we need to change how the process/procedure is
being done.

With the Histonet email, since people are constantly joining, often for a
day or two, we can't really improve the "training" aspect. Yes, there are
instructions when we first join, to print off/save how to subscribe or
unsubscribe or change personal information, etc. But (be honest) how many of
us pay attention to these types of instructions when we sign up to be a
"member" of a credit card or a on-line department store or an on-line book
store or other email lists? Most people do not. So we know that this type of
"training" is not effective. But we really can't do a one-on-one type of
training session for each person who signs on to Histonet. Therefore,
improving the training is not the answer.

The answer lies in modifying the process. Look at the bottom of those emails
from credit cards or hotels or department stores that you have signed up
with. There is usually a line that says "If you no longer wish to receive
these emails, click on this link and follow the instructions".

Add to that, various email lists have various methods on how to unsubscribe,
which can involve a link, or putting the word unsubscribe in the subject, or
putting the word unsubscribe in the message.

Histonet has a link at the bottom, but no instructions. So it's not clear to
click on the link to unsubscribe, nor is there any mention whether one of
the other unsubscribing methods would work. I therefore believe the Histonet
unsubscribing procedure has a process problem, that could be easily fixed.

As for the fact that how to unsubscribe has been explained 5,391+ times in
the past does not help the person who signed up over the weekend, and as of
today, decided that Histonet is not what they need. This new person has not
seen the previous requests for help with unsubscribing, nor the answers on
how to do it. Again, this is a process problem.

Is there any way Histonet can get some clearer instructions at the bottom of
each email, on how to unsubscribe, either permanently or temporarily while
on vacation? Such as saying "To unsubscribe, click on the link below, and
follow the instructions at the bottom of the next webpage".

Let's not yell at the people trying to unsubscribe. Let's work on improving
the unsubscribing process, so we don't get these requests.

Peggy A. Wenk, HTL(ASCP)SLS

-----Original Message-----
From: Manfre, Philip
Sent: Friday, September 06, 2013 7:30 AM
To: nmhisto <@t> comcast.net ; HISTONET
Subject: RE: [Histonet] Unsubscribe, Chapter 195

Trained professionals should know by now that if you want to unsubscribe,
you must type in all caps - UNSUBSCRIBE


Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre <@t> merck.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
nmhisto <@t> comcast.net
Sent: Thursday, September 05, 2013 9:57 PM
To: HISTONET
Subject: [Histonet] Unsubscribe, Chapter 195

It is a concern that members of our ??technically-oriented career field ??have
a difficult time understanding the method for unsubscribing to Histonet.
There is an ??almost- daily posting to "unsubscribe", despite the fact that
this subject has been addressed literally hundreds of times. ??When one
"joins" Histonet, instructions are provided, should be printed out for
reference and used if the subscriber decides to leave the group. ??We are
required to be knowledgeable on all manner of technical routines requiring
detailed instructions and ??Histonet is no less clear in the methods for
joining and "un-joining". ??Use them, please. ??Fire away - I'm retired and I
can take the flak! ??I do miss my microtome, though...
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------------------------------

Message: 9
Date: Mon, 9 Sep 2013 11:18:23 -0400
From: "Susan Foreman" <sforeman <@t> labpath.com>
Subject: [Histonet] ICC on previously Wright stained smears
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002101cead6f$d3a40540$7aec0fc0$@com>
Content-Type: text/plain;       charset="us-ascii"

Is anyone running ICC on previously Wright-Giemsa stained smears?  What kind
of info can you offer?   We didn't have any staining without antigen
retrieval, so we added that step.  So with the antigen retrieval,  the cells
are in good shape, but now there is too much background without very much
cellular staining.  What recommendations do you have?  Longer or shorter
time in antigen retrieval?  Adjust the pH of the antigen retrieval?  We
don't have very many smears left on this research patient.



Many Thanks,

Susan







------------------------------

Message: 10
Date: Mon, 9 Sep 2013 08:28:47 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] ICC on previously Wright stained smears
To: Susan Foreman <sforeman <@t> labpath.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1378740527.86695.YahooMailNeo <@t> web163106.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

You need antigen retrieval if you have a specimen that has been fixed with NBF but usually blood smears are just fixed with methanol and in this case antigen retrieval is not a necessary step.
Eliminate it and you will probably will get better results altogether.
Ren? J.


________________________________
From: Susan Foreman <sforeman <@t> labpath.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Monday, September 9, 2013 11:18 AM
Subject: [Histonet] ICC on previously Wright stained smears


Is anyone running ICC on previously Wright-Giemsa stained smears?? What kind
of info can you offer?? We didn't have any staining without antigen
retrieval, so we added that step.? So with the antigen retrieval,? the cells
are in good shape, but now there is too much background without very much
cellular staining.? What recommendations do you have?? Longer or shorter
time in antigen retrieval?? Adjust the pH of the antigen retrieval?? We
don't have very many smears left on this research patient.



Many Thanks,

Susan





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------------------------------

Message: 11
Date: Mon, 9 Sep 2013 08:46:29 -0700 (PDT)
From: dusko trajkovic <dunatrsd <@t> sbcglobal.net>
Subject: Re: [Histonet] Unsubscribe, Chapter 195
To: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1378741589.46291.YahooMailNeo <@t> web181705.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Peggy,
I don't think anyone else could have said it better.
Thank you
Dusko


________________________________
From: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>
To: "Manfre, Philip" <philip_manfre <@t> merck.com>; nmhisto <@t> comcast.net; HISTONET <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, September 9, 2013 7:47 AM
Subject: Re: [Histonet] Unsubscribe, Chapter 195


I'm going to wade in, not as someone who has posted numerous times on how to unsubscribe, but as someone assessing it from a "risk assessment" evaluation.

If there is a lab task that is consistently being done wrong, by many different people, it is usually NOT the fault of the people. It is either a training issue, or a process problem. So we either have to do a better job training and re-training, or we need to change how the process/procedure is being done.

With the Histonet email, since people are constantly joining, often for a day or two, we can't really improve the "training" aspect. Yes, there are instructions when we first join, to print off/save how to subscribe or unsubscribe or change personal information, etc. But (be honest) how many of us pay attention to these types of instructions when we sign up to be a "member" of a credit card or a on-line department store or an on-line book store or other email lists? Most people do not. So we know that this type of "training" is not effective. But we really can't do a one-on-one type of training session for each person who signs on to Histonet. Therefore, improving the training is not the answer.

The answer lies in modifying the process. Look at the bottom of those emails from credit cards or hotels or department stores that you have signed up with. There is usually a line that says "If you no longer wish to receive these emails, click on this link and follow the instructions".

Add to that, various email lists have various methods on how to unsubscribe, which can involve a link, or putting the word unsubscribe in the subject, or putting the word unsubscribe in the message.

Histonet has a link at the bottom, but no instructions. So it's not clear to click on the link to unsubscribe, nor is there any mention whether one of the other unsubscribing methods would work. I therefore believe the Histonet unsubscribing procedure has a process problem, that could be easily fixed.

As for the fact that how to unsubscribe has been explained 5,391+ times in the past does not help the person who signed up over the weekend, and as of today, decided that Histonet is not what they need. This new person has not seen the previous requests for help with unsubscribing, nor the answers on how to do it. Again, this is a process problem.

Is there any way Histonet can get some clearer instructions at the bottom of each email, on how to unsubscribe, either permanently or temporarily while on vacation? Such as saying "To unsubscribe, click on the link below, and follow the instructions at the bottom of the next webpage".

Let's not yell at the people trying to unsubscribe. Let's work on improving the unsubscribing process, so we don't get these requests.

Peggy A. Wenk, HTL(ASCP)SLS

-----Original Message----- From: Manfre, Philip
Sent: Friday, September 06, 2013 7:30 AM
To: nmhisto <@t> comcast.net ; HISTONET
Subject: RE: [Histonet] Unsubscribe, Chapter 195

Trained professionals should know by now that if you want to unsubscribe, you must type in all caps - UNSUBSCRIBE


Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre <@t> merck.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of nmhisto <@t> comcast.net
Sent: Thursday, September 05, 2013 9:57 PM
To: HISTONET
Subject: [Histonet] Unsubscribe, Chapter 195

It is a concern that members of our? technically-oriented career field? have a difficult time understanding the method for unsubscribing to Histonet. There is an? almost- daily posting to "unsubscribe", despite the fact that this subject has been addressed literally hundreds of times.? When one "joins" Histonet, instructions are provided, should be printed out for reference and used if the subscriber decides to leave the group.? We are required to be knowledgeable on all manner of technical routines requiring detailed instructions and? Histonet is no less clear in the methods for joining and "un-joining".? Use them, please.? Fire away - I'm retired and I can take the flak!? I do miss my microtome, though...
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Notice:? This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
New Jersey, USA 08889), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.






_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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------------------------------

Message: 12
Date: Mon, 9 Sep 2013 09:26:38 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] (no subject)
To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>,
        histonet-bounces <@t> lists.utsouthwestern.edu, "Naujokas,   Agne"
        <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier, Melissa"
        <Melissa.Meier <@t> ucsfmedctr.org>
Message-ID:
        <OFB1AEEE9E.B8E4DFFE-ON88257BE1.005A266D-88257BE1.005A38AD <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

Recommended melting point of paraffin is 2-4 degrees above the melting
point of the paraffin.  Because we really don't see paraffins that would
have a melting point of 46, the BEST answer would be 58-70.  Perhaps not
what we do, but the best answer for the choices provided.



From:   "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
To:     histonet <histonet <@t> lists.utsouthwestern.edu>
Cc:     "Naujokas, Agne" <Agne.Naujokas <@t> ucsfmedctr.org>, "Meier, Melissa"
<Melissa.Meier <@t> ucsfmedctr.org>
Date:   09/09/2013 06:30 AM
Subject:        [Histonet] (no subject)
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



Good morning all!

One of our fellows emailed me a question that she came across while
studying for her boards:



"I'm studying for my board exam and came across questions re: paraffin
embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or
higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting
point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but
we have also used paraffin that melts at 56C and we embedded at 58C.  Or
am I missing something?  Does anyone have a clear cut answer to this?



Thanks everyone!

Erin

Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service
415-353-7248

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------------------------------

Message: 13
Date: Mon, 9 Sep 2013 16:59:18 +0000
From: Sue Hunter <SHUNTER <@t> beaumont.edu>
Subject: RE: [Histonet] FISH enumeration
To: joelle weaver <joelleweaver <@t> hotmail.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <B493B3E4EF638A41875845CEDF938B440214D520 <@t> ExMail01.ms.beaumont.edu>
Content-Type: text/plain; charset="us-ascii"

My techs do the scoring for the FISH testing.  We have a Leica Ariol system - the HER2 FISH are computer assisted, but the Urovysions are manually assessed.  That may change with the new systems we are receiving.  My techs are exceptionally well trained in morphology - they sit with our director to learn, and are really really good. We do about 10 urovysions per week and sometimes as many as 30 pathvysions per week.  I have one or two techs on the FISH rotation each week.  The other techs step in as needed.  The pathologist circles the area on the HER2 IHC slide to be FISHed.  The Pathologists do come down to  the lab to see cases, but they rely on the techs.  The pathologists also look at all other criteria before signing out the case to make sure everything fits. We are also getting a new image hub so the saved images will be viewable by the pathologists - this will be a really nice addition to the reviewing process. I believe there are many laboratories where the cytotechs read out the urovsyions.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Saturday, September 07, 2013 10:22 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FISH enumeration

 For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification,  as well as an approximation of FTE's needed with some volumes. Do you do manual enumeration or use scanning software?
Thanks for any input.





Joelle Weaver MAOM, HTL (ASCP) QIHC

> From: TMcNemar <@t> lmhealth.org
> To: thisisann <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 6 Sep 2013 05:45:41 -0400
> Subject: RE: [Histonet] Cell Block Preparation
> CC:
>
> This is how we do it now.  In the old days, we used agar and to my mind, it is still the best way when you have scant material.
> - Spin in a conical tube and pour off
> - Melt an agar slant (we get TSA slant from micro)
> - Pour the agar into the conical tube and spin for 5 minutes
> - The agar will re-solidify and whatever sediment there is will be concentrated in the very tip of the cone
> - The agar will slide out of the centrifuge tube
> - Slice off the very tip and wrap in lens paper
> - Place the wrapped tip in a cassette and process as usual
> - Embed the specimen tip down and you are good to go...
>
> I still use this method today when I feel it necessary.  Works great.
>
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ann Specian
> Sent: Thursday, September 05, 2013 12:45 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Cell Block Preparation
>
>
> I am getting complaints in regard to "insufficient" cell blocks.  We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper.
>
> Does anyone have a more current technique which renders better cellularity?
>
> Also, do you know which renders a better cell block:  a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
>
> Thanks,
> Ann
> _______________________________________________
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>
> This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
>
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------------------------------

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End of Histonet Digest, Vol 118, Issue 10
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