[Histonet] (no subject)

Manfre, Philip philip_manfre <@t> merck.com
Mon Sep 9 08:42:48 CDT 2013

It's a bad question.  Generally the "best" temperature is probably between 55 and 65, depending on the paraffin.  This would fall between the two latter answers.  Hmm....  What to do?


Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
PO Box 4
West Point, PA 19486

215-993-0383 (fax)
philip_manfre <@t> merck.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Monday, September 09, 2013 9:28 AM
To: histonet
Cc: Naujokas, Agne; Meier, Melissa
Subject: [Histonet] (no subject)

Good morning all!

One of our fellows emailed me a question that she came across while studying for her boards:

"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"

This seems to me to be an odd question because it depends on the melting point of the paraffin in use.  Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C.  Or am I missing something?  Does anyone have a clear cut answer to this?

Thanks everyone!


Erin Martin, Histology Supervisor

UCSF  Dermatopathology Service

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