[Histonet] Cell Block Preparation
Richard.Cartun <@t> hhchealth.org
Sun Sep 8 19:20:12 CDT 2013
We borrowed a wonderful cell block procedure from Windham Community Memorial Hospital here in CT several years ago. The specimen is collected fresh in sterile saline or RPMI and then centrifuged to concentrate the cells. Plasma and then thrombin are added to form a clot. The clot is then fixed in formalin and processed in our Histology Laboratory. I will send the procedure to anyone who is interested.
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Ann Specian [thisisann <@t> aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to "insufficient" cell blocks. We currently spin, pour off the supernatant, retrieve the sediment and process in lens paper.
Does anyone have a more current technique which renders better cellularity?
Also, do you know which renders a better cell block: a fresh specimen, a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
More information about the Histonet