using celloidin RE: [Histonet] Cell Block Preparation
Timothy.Morken <@t> ucsfmedctr.org
Fri Sep 6 11:48:55 CDT 2013
Our cytology department uses a technique that involves coating a centrifuge tube with Celloidin then spinning the sample in that tube. The cellodin is then scored and the "bag" of celloidin containing the cell button is taken out and wrapped in paper to be processed in a cassette. It is a tricky and a bit time consuming, but no material is lost in processing. We do up to 10 samples a day that way.
J Clin Pathol. 1982 May; 35(5): 574-576.
A celloidin bag for the histological preparation of cytologic material.
Department of Pathology
UC San Francisco Medical Center
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J
Sent: Friday, September 06, 2013 9:39 AM
To: Ann Specian; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation
We have had mixed results in the past with cell blocks, but currently we are filtering the fluid through a biopsy bag and processing that. We seem to be retaining more specimen with that method (we have used sponges and lens paper in the past).
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526
From: Ann Specian [mailto:thisisann <@t> aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to "insufficient" cell blocks. We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.
Does anyone have a more current technique which renders better
Also, do you know which renders a better cell block: a fresh specimen,
a specimen fixed in Cytolyt or a specimen fixed in 10% NBF?
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom
they are addressed.
If you have received this email in error please notify the
originator of the message. This footer also confirms that this
email message has been scanned for the presence of computer viruses.
Any views expressed in this message are those of the individual
sender, except where the sender specifies and with authority,
states them to be the views of Commonwealth Health.
Scanning of this message and addition of this footer is performed
by Websense Email Security software in conjunction with
virus detection software.
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet