[Histonet] Eosin problem

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Sep 3 16:47:19 CDT 2013

Firstly: you should not schedule changing the eosin or any stain for that matter on a "time" (in this case) basis. The reagents ought to be changed on usage basis, i.e. establish a maximum slides to be stained before changing the reagents.
Second: your pathologists are wrong because had the time in formalin any bearing on the problem, all the racks should come pale and not only the first.
Third: it is very likely that during the weekend some sort of surface oxidation may occur and that is why stirring the eosin "solves" the problem.
Four: Faced with this problem and if you insist on changing the reagents of a time basis, change them on Mondays even if somebody has to come on the weekend to section/stan
René J.

From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
To: "Histonet Post (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Tuesday, September 3, 2013 1:09 PM
Subject: [Histonet] Eosin problem

Hi All,'

Hoping someone can give me some insight on if this happens/ happened to them.  We change all of our reagents on the H&E stainer on Friday( which means the reagents sit idle over the weekend.)  We rarely have to come in on the weekends due to having to embed/cut/stain tissues.  However, on Monday or in this case today (Tues because of the Holiday), we have to stir our Eosin before putting our first rack through for staining because the Pathologists have been complained that the Eosin is too light if we don't.  But I've had one of them tell me that a second and subsequent rack of slides does'nt have this problem.  The Pathologist wants to know if by the tissue sitting in Formalin longer over the weekend on the processor vs. only sitting a few extra hours on the overnight run, is the extra time in Formalin causing this problem?
I told him that I did'nt think so, but that I would ask.  I also thought that maybe in addition to stirring the Eosin, maybe running a rack of "blank" slides through a staining program before puttng the patient tissue through might help.  What do you all think??

Thanks so much!!

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com

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