[Histonet] RE: cell block staining issues

[Martha Ward-Pathology]

Well actually it does help because we are trying to eliminate possible causes and it is nice to know that someone else uses Cytolyt with no problems.   It is just frustrating because we are doing nothing different that I can find.    The next step is to see if the samples are coming from the same doctor/clinic and see if they are doing anything different.

Martha

-----Original Message-----
From: Tom McNemar [mailto:TMcNemar <@t> lmhealth.org] 
Sent: Thursday, October 31, 2013 9:17 AM
To: Martha Ward-Pathology; histonet <@t> lists.utsouthwestern.edu
Subject: RE: cell block staining issues

We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues.  Sorry, I know this isn't much help.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology
Sent: Thursday, October 31, 2013 8:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cell block staining issues



We have something of a mystery here and I am hoping someone can help.  My cytopath docs are saying that our B72.3 (TAG-72) antibody is not working well in cell blocks created from fluids (plural), etc. and that this is a fairly recent development (over the past 5-6 weeks or so).    The surgical cases we have stained look great as does our control, which is placed on each slide.   We have not changed lot numbers of antibody or made any up recently and the antibody is not expired, or even close.    The only thing we can find that is different is that cytology has been rinsing the needles out with a product called Cytolyt, instead of saline and they started this around the first of August.    Does anyone know if this product could cause any interference with our staining?  The docs just say that the tumor cells are not staining and we are sort of at a loss here.

Thanks in advance for your help.
Martha Ward, MT (ASCP) QIHC
Manager
[Wake Forest Baptist Health]
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.2109  \  f 336.716.5890 mward <@t> wakehealth.edu



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