[Histonet] cutting thick brain frozen sections for Golgi stain

[Grantham, Andrea L - (algranth)]

I need to cut 80 micron sections of frozen brain.
Tried today at -18 degrees C but the brains kept cracking. I read that the temp should be -3 to -13 degrees C. Has anybody cut sections this thick of brain? Can you pass along any words of inspiration?



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097