[Histonet] RE: Fixation Question

[Cesar Francisco Romero]

The total Osmolarity of the
fixing solution should be isosmotic with tissue fluids in order to prevent
shrinkage or swelling of cells. 

The best way to avoid shrinkage
is to prepare Formalin with Physiological or Isotonic Saline Solution.

The solution is 9 grams of sodium chloride ( NaCl )
dissolved in Distilled Water, to a total volume of 1000 ml.

To prepare the fixative use 100 ml of pure Formalin (
Formalin 37 ~ 40 % ) and 900 ml of Isotonic Saline Solution.


Cesar Romero
Buenos Aires
Argentina