[Histonet] Re: India Ink for marking surgical margin borders

[Barry Rittman]

Bob
Good point - it is true that they are not in particles when used as a
solution - but the colors can also be seen by visible light as well as by
fluorescence.
When I used to mark  biopsy specimens it was mainly to allow correct
orientation of the block in the wax and to allow the  marked area to be
seen when orienting and cutting the block rather than visualizing in a
section.
Barry



On Sat, Oct 12, 2013 at 12:15 PM, Bob Richmond <rsrichmond <@t> gmail.com> wrote:

> Barry Rittman observes: >>One group of dyes that I do not believe has ben
> mentioned is the Procion group of dyes. These are chloro-s-triazone dyes
> that are used extensively in textile dyeing and are resistant to all the
> solvents of which I am aware and will survive processing through paraffin
> wax.  Readily available at many art stores. They come in a wide variety of
> colors. They interact with proteins. Their drawback is that many are
> fluorescent (and were originally intended as markers for hard tissue growth
> due to their solvent resistance).<<
>
> But if they aren't in insoluble particles, then I won't be able to see them
> under the microscope!
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
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