[Histonet] RE: Negative Reagent Controls in Diagnostic IHC....

[Sebree Linda A]

Richard,

I wish we could eliminate them but not only do our negatives displaying some non-specific staining, seems to be tissue dependent, but our in-house QA people say we need to continue using them per the data sheets accompanying many of our antibodies.  We use Ventana UltraView DAB detection, a multimer kit.

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cartun, Richard
Sent: Wednesday, October 09, 2013 3:59 PM
To: Histonet
Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC....

My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, "Negative Reagent Controls in Diagnostic Immunohistochemistry:  Do we need them?".  I have received a few requests for the actual poster (PowerPoint slide).  I will be happy to e-mail it to anyone who is interested.



Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year!



Richard



Richard W. Cartun, MS, PhD

Director, Histology & Immunopathology

Director, Biospecimen Collection Programs

Assistant Director, Anatomic Pathology

Hartford Hospital

80 Seymour Street

Hartford, CT  06102

(860) 545-1596 Office

(860) 545-2204 Fax

richard.cartun <@t> hhchealth.org<mailto:richard.cartun <@t> hhchealth.org>

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