[Histonet] Vacuum and pressure in tissue processing

[Rene J Buesa]

When tissue processing was manual there were some "gadgets" providing vacuum and those using it reported better results. The fact of the matter was that manual processing is so slow that anything you introduce will favor the process.
Static tissue processors, i.e. those that only mover the specimens circumventing the manual transfer like the HistoKinete only improved processing if they were able to move the specimens, something they did by adding rotation inside the reagents vessels.
Retort tissue processors introduced 3 novelties: vacuum, pressure and, most importantly, agitation that is nothing but empty/fill the whole retort every 20 minutes. This agitation is more important, as you point out, than the vacuum/pressure.
René J.


________________________________
From: Teri Johnson <TJohnson <@t> gnf.org>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Wednesday, October 2, 2013 6:57 PM
Subject: [Histonet] Vacuum and pressure in tissue processing


Dear friends,

I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more activity kinetically and therefore makes processing more efficient.

Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue processing, please?

Thank you, as always, for your wisdom.

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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