[Histonet] HT HistoDeck question...
Stephenson, Sheryl
SStephenson <@t> lifecell.com
Thu Oct 3 09:02:56 CDT 2013
Thank you All!
Sheryl Stephenson | Histology Technician
-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu]
Sent: Thursday, October 03, 2013 9:43 AM
To: Watson, Linda; Lee & Peggy Wenk; Stephenson, Sheryl; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...
We fix H&E's in 95% and our IHC protocol is acetone/alcohol fixation.
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Watson, Linda
Sent: Thursday, October 03, 2013 8:40 AM
To: Lee & Peggy Wenk; Stephenson, Sheryl; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] HT HistoDeck question...
For frozen cut sections, would the fixation also depend on what you plan on doing with it. For example, H&E, Special Stain or IHC? Please correct if I am wrong. I think that is a trick question!!!
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-
>bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk
>Sent: Thursday, October 03, 2013 8:16 AM
>To: Stephenson, Sheryl; histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] HT HistoDeck question...
>
>Personally, I think it's "a" is a wrong answer, and that you are
>correct that "b" is a better answer. My students and I have found a
>couple of other questions that we thought had the wrong answer
>indicated in the study set.
>
>Peggy A. Wenk, HTL(ASCP)SLS
>-----Original Message-----
>From: Stephenson, Sheryl
>Sent: Thursday, October 03, 2013 7:21 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] HT HistoDeck question...
>
>Hi,
>Please clarify why this answer to the HistoDeck study question is a)
>and not b).
>
>Here is the question:
>
> 'Frozen section slides cut from fresh, unfixed tissue specimens are
>optimally fixed in which of the following solutions?
>a) 37%-40% formaldehyde
>b) Cold acetone
>c) Acetic acid alcohol
>d) Alcoholic formalin
>
>Thanks,
>
>
>Sheryl Stephenson | Histology Technician
>
>
>
>
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