[Histonet] Vacuum and pressure in tissue processing

[Teri Johnson]

Dear friends,

I recall hearing at a conference (or maybe it was just a casual conversation by an expert during a NSH symposium break) that vacuum and pressure in tissue processing really accomplishes very little. I do believe that using heat and agitation of the solutions provides more activity kinetically and therefore makes processing more efficient.

Can someone affirm or deny the efficacy of vacuum and/or pressure in tissue processing, please?

Thank you, as always, for your wisdom.

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752