[Histonet] Thanks! RE: thawing and refreezing muscle?

[Morken, Timothy]

Thanks to everyone for their muscle refreezing methods. We ended up being able to quickly split the samples and cut without thawing.  But we will try some out for the future, just in case.


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center





-----Original Message-----
From: Hawkins, Hal K. [mailto:hhawkins <@t> UTMB.EDU] 
Sent: Tuesday, October 01, 2013 1:35 PM
To: Walter Benton; Morken, Timothy; Histonet
Cc: jmitchell <@t> neurology.wisc.edu
Subject: RE: thawing and refreezing muscle?


We have had reasonable results based on rapid thawing of frozen samples in room temperature buffer followed by formalin fixation or rapid freezing in OCT.  With such large samples, though, there are bound to be large ice crystals in the interior of the specimen regardless of the method used for freezing. 

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Walter Benton [wbenton <@t> cua.md]
Sent: Tuesday, October 01, 2013 12:46 PM
To: Morken, Timothy; Histonet
Cc: jmitchell <@t> neurology.wisc.edu
Subject: [Histonet] RE: thawing and refreezing muscle?

This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things.

http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf

http://www.lifebloodmedical.com/

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken <@t> ucsfmedctr.org]
Sent: Tuesday, October 01, 2013 12:07 PM
To: Histonet
Cc: jmitchell <@t> neurology.wisc.edu
Subject: [Histonet] thawing and refreezing muscle?

Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center

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