[Histonet] RE: thawing and refreezing muscle?

[Laura Avogaro]

I would like to fix my muscle tissue samples in formalin after freezing in
OCT. Does anyone have experience or could give me some suggestion? I would
like to avoid artifacts...


Laura Avogaro
Centre for Integrative Biology
University of Trento
Via delle Regole, 101 38123 Mattarello (TN) – Italy




>
> We have had reasonable results based on rapid thawing of frozen samples
in
> room temperature buffer followed by formalin fixation or rapid freezing
in
> OCT.  With such large samples, though, there are bound to be large ice
crystals in the interior of the specimen regardless of the method used
for
> freezing.
>
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Walter Benton
[wbenton <@t> cua.md]
> Sent: Tuesday, October 01, 2013 12:46 PM
> To: Morken, Timothy; Histonet
> Cc: jmitchell <@t> neurology.wisc.edu
> Subject: [Histonet] RE: thawing and refreezing muscle?
>
> This may help. Never used it when I processed fresh/frozen muscle
specimens but have heard good things.
>
> http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf
>
> http://www.lifebloodmedical.com/
>
> Walter Benton HT(ASCP)QIHC
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> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
[Timothy.Morken <@t> ucsfmedctr.org]
> Sent: Tuesday, October 01, 2013 12:07 PM
> To: Histonet
> Cc: jmitchell <@t> neurology.wisc.edu
> Subject: [Histonet] thawing and refreezing muscle?
>
> Histonetters, Does anyone have a good method for thawing muscle and
refreezing for histochemistry? With good results? We have some
bulk-frozen
> muscle (centimeter thick) and that is what they want to try.
>
> Tim Morken
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
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