[Histonet] RE: thawing and refreezing muscle?

[Walter Benton]

This may help. Never used it when I processed fresh/frozen muscle specimens but have heard good things.

http://www.cell-ess.com/Nonfrozen_Transport_Medium_Preserves_and_Restores_Skeletal_Muscle_Enzymatic_Activity_and_Morphology.pdf

http://www.lifebloodmedical.com/

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
ChesapeakeUrology.com

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________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Morken, Timothy [Timothy.Morken <@t> ucsfmedctr.org]
Sent: Tuesday, October 01, 2013 12:07 PM
To: Histonet
Cc: jmitchell <@t> neurology.wisc.edu
Subject: [Histonet] thawing and refreezing muscle?

Histonetters, Does anyone have a good method for thawing muscle and refreezing for histochemistry? With good results? We have some bulk-frozen muscle (centimeter thick) and that is what they want to try.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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