[Histonet] Validating new Benchmark instrument

[Fawn Bomar]

Hi everyone,



I know that this subject has come up several times and I am sorry to bother everyone again but I was hoping to find some help.  We are upgrading our Benchmark Immuno machines to the Benchmark XT immuno machine at the end of October.  Does anyone have any suggestions on how to approach the validation process?  Do we need to validate every antibody that we use as well as the kits?  I know that there is also a gray area surrounding how many slides need to be run to consider the antibody validated, but what would be the suggested amount of slides to validate a new machine as I know this will be very costly.  Does anybody have a log/checklist/spreadsheet that they would be willing to share to help document this validation of the machine and each of the antibodies?



Thank you in advance for your help



Fawn Bomar
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Thank you