[Histonet] human lymph nodes from a cadaver

[Scanlan, Megan Anne]

Yup, that's standard with our cutting here but I will try giving them a lot more time on the ice bath once faced.  Also at Linda Blazek we processed and embedded their tissue samples, so it's is already in the paraffin we use.

Thank you,
   Megan


Megan Scanlan
Research Technician I
Wellman Center for Photomedicine
Massachusetts General Hospital
50 Blossom Street, EDW 214
Boston, MA 02114
617-726-6983 (Lab)
726-1206 (fax)

-----Original Message-----
From: Britton, Josette C [mailto:JCBRITTON <@t> Cheshire-Med.COM] 
Sent: Tuesday, October 01, 2013 7:41 AM
To: Scanlan, Megan Anne
Subject: RE: [Histonet] human lymph nodes from a cadaver 

Face your blocks and soak them well in an ice water bath!

Josie Britton HT (ASCP) QIHC
Cheshire Medical Center
Keene, NH 03431

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scanlan,
Megan Anne
Sent: Monday, September 30, 2013 3:52 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] human lymph nodes from a cadaver 


Hi all,

Does anyone have experience cutting human lymph nodes from a cadaver in
paraffin blocks?  I am having quite a lot of trouble getting good
sections, even using a brand new blade the sections end up with multiple
blade marks streaking through the tissue and paraffin.

I've contacted the researcher that gave me the samples to cut asking
about how the cadaver was preserved and what fixatives they used.  I'm
thinking that the samples may not have been processed well enough since
I've tried all the normal tricks for cutting paraffin's.  There was one
sample that seemed to have a calcium deposit or some very hardened
tissue, not entirely sure but it nicked up the blade pretty good making
sectioning impossible on that one.

I'm not sure if the reason I am having trouble sectioning is because
these came from a cadaver or because they are human lymph nodes, it is
my first time cutting either of these things.

Any and all advice and opinions are welcomed

Thank you,
     Megan


Megan Scanlan
Research Technician I
Wellman Center for Photomedicine
Massachusetts General Hospital
50 Blossom Street, EDW 214
Boston, MA 02114
617-726-6983 (Lab)
781-635-6626 (Cell
726-1206 (fax)




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