R: RE: [Histonet] Over fixation

[massimo.tosi_m <@t> libero.it]

It is an ancient method but it works well to avoid that the formalin becomes 
acid. 
It owed to the formation of formic acid (HCOOH).
It is enough to put i a little piece of marble (CaCO3) nto the solution 
together with the specimens.
(Reference:" Histology Technique" - Dr. Hans-Christian Burck)

Kind Regards,
Massimo Tosi

>----Messaggio originale----
>Da: Tony.Reilly <@t> health.qld.gov.au
>Data: 25-nov-2013 0.37
>A: "Sanjeet"<asanjeet <@t> yahoo.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
>Ogg: RE: [Histonet] Over fixation
>
>Hi Sanjeet
>
>It is true that tissue can bleach over time in formalin making staining 
difficult.  This possibly happens if the formalin becomes acidic over time.  
This can be improved by treating the tissue with 0.05% periodic acid followed 
by a water wash and then staining as usual.
>
>Regards
>Tony
>
>  
>
>Tony Reilly  B.App.Sc. , M.Sc.
>
>Chief Scientist, Anatomical Pathology
>
>Pathology Queensland-PA Laboratory 
>
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>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-
bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sanjeet
>Sent: Monday, 25 November 2013 6:33 AM
>To: Tony Reilly; histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Over fixation
>
>Hi Histo folks,
>Tissue biopsy that are  not fixed properly do have an adverse effect on H & E 
staining. Tissues can be kept in formalin for a period of 5 to 7 days as per 
literature. But what happens to the tissues that are kept over for a longer 
period of time. By that I mean 6 to 12 months. Would over fixed tissues have 
any effect on H & E stain. Some of my colleagues say NO, but I say YES. I find 
that the over fixed tissues lose their affinity towards the dyes . Please 
advice. 
>
>Sent from my iPhone
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