[Histonet] RE: Esophageal Brushings

Fri Nov 22 07:21:01 CST 2013

Debbie's method would work very well but in our experience with cytospins, if the fixation doesn't occur prior to the cells being applied the slide but after, you can end up with air drying artifact on the slides you have destined for the Pap stain.  If your pathologist want only Pap stained slides, then you could add a small amount cytospin collection fluid to the button, then add a few drops to your cytospin apparatus.  There is one caution if you do add this fluid.  The fluid contains a carbomer that must be dissolved by soaking in 95% ETOH prior to performing the Pap stain.  The carbomer helps protect the cells from air drying and it also helps the cells adhere to the slide.

In our method, we follow Debbie's but we add a couple of drops of unfixed, resuspended cells from the button to 1 apparatus and then add fixative to the button for adding to the second apparatus.  This way we get a slide for the Pap stain and then a slide for a Romanowsky (we use May-Grunwald Geimsa) stain.  As Debbie has pointed out, the unfixed slide can also be used for immunostains but we rarely perform them on GI brushing specimens at our institution.  This is how our pathologist perform to review these cases when we perform cytospins on cases.  With that said, we now primarily utilize the ThinPrep system for most of our Non-GYN specimens.  This would probably be an expensive option if you are only collecting esophageal brushings.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewalker <@t> rrmc.org    www.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor's Award for Performance Excellence

-----Original Message-----
From: Boyd, Debbie M [mailto:DKBoyd <@t> chs.net]
Sent: Friday, November 22, 2013 7:52 AM
To: Joe W. Walker, Jr.; Amber McKenzie
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: Esophageal Brushings

We receive the brushes in saline.  We vortex the brush (in the saline) to dislodge any cells attached to it.  Spin down the specimen, pipette off the supernant, and make 2 cytospins from the button.  You can than perform your stain.  There isn't any fixative at this point.  For cyptologic evaluation you need to fix them immediately in 95% EtOH .  For IHC stains air dry them.

-----Original Message-----
From: Joe W. Walker, Jr. [mailto:joewalker <@t> rrmc.org]
Sent: Thursday, November 21, 2013 4:38 PM
To: Boyd, Debbie M; Amber McKenzie
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: Esophageal Brushings

Wow are the brushes sent to you?  In a collection medium like cytorich red or saline?  Are slides prepared with the brush and fixed or air dried?  Cytospins could work and a Pap stain would be preferred on a fixed slide but perhaps your pathologist want a Romanowsky type stained slide too since air dried slides can show things that a fixed slide will not.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewalker <@t> rrmc.org    www.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, November 21, 2013 3:38 PM
To: Amber McKenzie
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Esophageal Brushings

We make 2 cytospins and stain them with the Pap stain.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, November 21, 2013 12:24 PM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Esophageal Brushings

Do any of you do Esophageal Brushings in your lab?  If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer?  Who do you order your supplies from?  There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me.
Thanks!

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