[Histonet] RE: Esophageal Brushings

[Joe W. Walker, Jr.]

Wow are the brushes sent to you?  In a collection medium like cytorich red or saline?  Are slides prepared with the brush and fixed or air dried?  Cytospins could work and a Pap stain would be preferred on a fixed slide but perhaps your pathologist want a Romanowsky type stained slide too since air dried slides can show things that a fixed slide will not.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewalker <@t> rrmc.org    www.rrmc.org

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Thursday, November 21, 2013 3:38 PM
To: Amber McKenzie
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Esophageal Brushings

We make 2 cytospins and stain them with the Pap stain.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Thursday, November 21, 2013 12:24 PM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Esophageal Brushings

Do any of you do Esophageal Brushings in your lab?  If so, what do you use to stain the slides...just coplin jars side by side dipping the slides in or some type of stainer?  Who do you order your supplies from?  There were a couple of stains I didn't recognize in the protocol we are supposed to use. This is all new to me.
Thanks!

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