[Histonet] Second Question of the Day: -80 degrees rationale

[Paula Sicurello]

Hello Again,
Gayle and Bill have provided the most succinct answers to the question of
the day.
Second Question: Is there a lower end, say -70 or -75, that provides the
same protection as -80?

I'm asking all this because I've inherited an old -80 freezer and would
like it to last as long as possible.  The lab that had this freezer
previously had an acceptable range of -70 to -90.

Thanks in advance, again, oh wise ones.
Paula
 --
> Paula Sicurello, HTL (ASCP)
> Supervisor, Clinical Electron Microscopy Laboratory
> Duke University Health System
> Rm.#251M, Duke South, Green Zone
> Durham, North Carolina 27710
> P: 919.684.2091
>
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On Wed, Nov 20, 2013 at 10:58 AM, WILLIAM DESALVO
<wdesalvo.cac <@t> outlook.com>wrote:

> Here is my stab at why -80 C.
>
> Temperatures between 0°C and -25°C, the enzymatic activity of cells is
> only slowed but remains active. Below -40°C physiochemical exchanges are
> frozen. Cellular morphology is preserved at -80°C. Shelf life of tissue
> increases as the temperature drops. Once you get below -80°C you will
> need cryoprotectors and when you use cryoprotectors, temperatures must be
> below -130°C and will go as low as -196°C (liquid nitrogen).  Antibodies
> and proteins in solution are stable at -20°C.
>
> *William DeSalvo,* *BS HTL(ASCP)*
>
> > Date: Wed, 20 Nov 2013 09:25:54 -0500
> > From: patpxs <@t> gmail.com
> > To: histonet <@t> lists.utsouthwestern.edu; Microscopy <@t> microscopy.com
> > CC:
> > Subject: [Histonet] -80 degrees rationale
>
> >
> > Hello My Fellow Listers,
> >
> > The question of the day is: What is the rationale for storing frozen
> > biopsies at -80 degrees?
> >
> > I have seen protocols that range in temperature from -40 to -80 degrees.
> >
> > Was -80 selected because that was the lowest freezers could go back in
> the
> > day?
> >
> > Awaiting your chilly responses!
> >
> > Thanks in advance,
> >
> > Paula
> >
> >
> > _______________________________________________
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> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

HIPAA Privacy Notification: This message and any accompanying documents are
covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521,
and contain information intended for the specific individual (s) only. This
information is confidential. If you are not the intended recipient or an
agent responsible for delivering it to the intended recipient, you are
hereby notified that you have received this document in error and that any
review, dissemination, copying or the taking of any action based on the
contents of this information is strictly prohibited . If you have received
this communication in error, please notify us immediately by e-mail, and
delete the original message.