[Histonet] -80 degrees rationale

WILLIAM DESALVO wdesalvo.cac <@t> outlook.com
Wed Nov 20 09:58:42 CST 2013

Here is my stab at why -80 C.
Temperatures between 0°C and −25°C, the enzymatic activity of cells is only slowed but remains active. Below −40°C physiochemical exchanges are frozen. Cellular morphology is preserved at -80°C. Shelf life of tissue increases as the temperature drops. Once you get below -80°C you will need cryoprotectors and when you use cryoprotectors, temperatures must be below −130°C and will go as low as −196°C (liquid nitrogen).  Antibodies and proteins in solution are stable at −20°C.

William DeSalvo, BS HTL(ASCP)

> Date: Wed, 20 Nov 2013 09:25:54 -0500
> From: patpxs <@t> gmail.com
> To: histonet <@t> lists.utsouthwestern.edu; Microscopy <@t> microscopy.com
> CC: 
> Subject: [Histonet] -80 degrees rationale
> Hello My Fellow Listers,
> The question of the day is:  What is the rationale for storing frozen
> biopsies at -80 degrees?
> I have seen protocols that range in temperature from -40 to -80 degrees.
> Was -80 selected because that was the lowest freezers could go back in the
> day?
> Awaiting your chilly responses!
> Thanks in advance,
> Paula
> -- 
> Paula Sicurello, HTL (ASCP)
> Supervisor, Clinical Electron Microscopy Laboratory
> Duke University Health System
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