[Histonet] coverslipping question

Kim Donadio one_angel_secret <@t> yahoo.com
Wed Nov 13 13:35:58 CST 2013

I would try adding 1 or 2 more 100% alcohols before the histoclear. 

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On Nov 12, 2013, at 9:32 AM, "Gautier, Nicole M." <ngauti <@t> lsuhsc.edu> wrote:

> My lab has been having a problem with "specks" appearing on our slides after they come out of Histoclear.  Our protocol is to dehydrate in 2 minutes each of 70%, 80%, 95%, and 100% ethanol before 2 minutes 2 times in Histoclear.  The slides are perfectly clear when they come out of the ethanols, but not when they come out of the Histoclear.
> Since it's not a problem we had when we first started, I tried changing out the Histoclear, but the problem remained.  At the end of last week, I changed out everything - all glassware, all ethanols, all Histoclear.  The problem went away and the 24 slides looked fine.  Today, the slides are looking speckled again.
> So I guess my questions are: What could be causing the speckled appearance of the slides? and  How often should I have to change the ethanols and Histoclear?  I seem to remember years ago when I did this that we only changed the solutions monthly...
> Nicky Gautier
> Research Associate
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