[Histonet] PicroSirius Red in Frozen Sections
avogaro <@t> science.unitn.it
Tue Nov 12 02:01:37 CST 2013
Dear Yoanna I performed Picrosirius Red staining on my tissue frozen
sections with good results so I suggest you operate as follows:
1)Fix slide in 4% PFA (5 min);
2)Rinse in ddH2O;
3)Incubate in Phosphomolybdic Acid (2 minutes);
4)Rinse in ddH2O;
5)Incubate in Picrosirius Red for (1 h);
6)Wash in acidified water (2X, 1 min each);
7)Rinse in 70% EtOH (30);
8)Dehydrate through ascending grades of alchol(80%, 90%, 100%);
9)Wash on Xilene (2X);
Good luck and let me know!
> I did a Picrosirius red stain using the polysciences kit in paraffin
section cut at 4microns. The stain looks great. Later on my boss asked
> to do the picro using the same kit but in frozen section. The stain
> very, very intense. Sections are really red. When looking under the
brightfield microscope there is not yellow, the entire tissue is red.
Under the polarized microscope, we can see the fiber but no like the
paraffin sections. Does anybody have come across this situation before?
Does the picrosirius stain only works for paraffin sections? I will
appreciate any feedback on the matter. I have been searching online for
> protocol of Picrosirius stain for frozens, but no luck.
> Thank you in advance,
> Yoanna Bello
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