[Histonet] Nail processing

WILLIAM DESALVO wdesalvo.cac <@t> outlook.com
Fri Nov 8 11:15:05 CST 2013

This process has worked for us for years. I strongly suggest the Fisher Brand Burnt Orange Plus Slides. We have tried them all and consistently the Burnt Orange work the best. We use them when we really need something to stick to the slide. Can't scientifically explain, just lots of testing and use.

Grossing Sections for Histology:Complete 10% Formalin FixationNails are soaked in a 10% solution of
potassium hydroxide.Hard nails may require 30-45 minutes.  Softer nails should be soaked for 10-30
minutes.Over soaking can destroy the
specimen.Rinse and place in Formalin to wait for processing.Submit one cassette of representative

Microtomy of SectionsUse Fisher Brand Burnt Orange Plus
Slides.Cut two sections per slide.Air Dry then place in 60⁰ C oven for 2
hours.Allow to come to room temp before starting staining process.

William DeSalvo, BS HTL(ASCP)
Production Manager-Anatomic Pathology
Chair, NSH Quality Management Committee
Owner/Consultant, Collaborative Advantage Consulting

 > From: Allison.Scott <@t> harrishealth.org
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Fri, 8 Nov 2013 16:58:30 +0000
> Subject: [Histonet] Nail processing
> Happy Friday to all.  I hope everyone has a great weekend.  I am I need of a procedure for processing nails.  We are having problems with keeping the nails on during gms special staining.  We are using the ventana machine not by hand.  I remember a procedure that used nair, it has been along time and I cant remember.  Any help will be appreciated.
> Allison Scott HT(ASCP)
> Supervisor, Histology Lab
> LBJ Hospital
> Harris Health System
> Office: 713-566-2148
> Lab: 713-566-5287
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