[Histonet] CD 34 antibody abcam (ab963)

pruegg <@t> ihctech.net pruegg <@t> ihctech.net
Wed Nov 6 20:29:45 CST 2013

This is a tuff one CD34 will stain all precursor cells not just endothelial cells for vessels, there is an excellent CD31 for ms tissue from Dionova it is rat anti ms but not sure of what you could use for cd31 for rat and rabbits, but I know cd34 is not the way to go.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com 
rueggihcconsultingpr <@t> outlook.com


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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of tahseen
Sent: Wednesday, November 06, 2013 5:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD 34 antibody abcam (ab963)

Hi All,
We used CD 34 antibody abcam (ab963)with detection kit enVision to stain our samples from rats and rabbits. We need to stain vessels only but macrophages, fibroblast, collagen all are stained in other hand known +ve control (Tonsil) is perform good.  Please help us that what should we do for IHC staining of our samples.
Best regards
Muhammad Tahseen
Sr.Supervisor Histology

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