[Histonet] FW: Fungus Controls

Tom McNemar TMcNemar <@t> lmhealth.org
Wed Nov 6 04:37:13 CST 2013

I made some controls years ago that we are still using today.  I used bread mold and streaked it out on a blood agar plate.  Once the plate was covered, cut the agar into cubes, wrapped it in lens paper and processed.  Cuts and stains beautifully.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Tuesday, November 05, 2013 2:04 PM
To: histonet <@t> lists.utsouthwestern.edu; Vickroy.Jim <@t> mhsil.com
Subject: [Histonet] FW: Fungus Controls

I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains.  Its "naturally occurring", a petri dish would not be naturally occurring in my mind.

Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it.  Actually happy I was able to save some money.

Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!!  I have never tried that, but if it works that would be awesome.   Thanks,

Timothy N. Higgins, HT (ASCP), QIHC

> Message: 4
> Date: Mon, 4 Nov 2013 15:07:06 -0500
> From: Patrick Laurie <foreightl <@t> gmail.com>
> Subject: Re: [Histonet] Aspergillus tissue blocks for controls
> To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
>       <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>       <CAKEyg-2KaDkn0oFQURzuPcugQt=Ju8LKK6C8daH+nPjvVsPMJw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> Hi Jim,
> I have found a couple of ways.  First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic).  Another method, which doesn't work the best,  can be
> moldy orange peel.  And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
> Good luck!
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:
> > We are in desperate needs of obtaining GMS controls.   In the old days we
> > had all the fungal specimens we needed.  Today it is getting hard to find
> > these tissues or controls.   Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> >
> > James Vickroy BS, HT(ASCP)
> >
> > Surgical  and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
> >
> >
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