[Histonet] FW: Fungus Controls
TMcNemar <@t> lmhealth.org
Wed Nov 6 04:37:13 CST 2013
I made some controls years ago that we are still using today. I used bread mold and streaked it out on a blood agar plate. Once the plate was covered, cut the agar into cubes, wrapped it in lens paper and processed. Cuts and stains beautifully.
Tom McNemar, HT(ASCP)
Licking Memorial Health Systems
tmcnemar <@t> lmhealth.org
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Tuesday, November 05, 2013 2:04 PM
To: histonet <@t> lists.utsouthwestern.edu; Vickroy.Jim <@t> mhsil.com
Subject: [Histonet] FW: Fungus Controls
I have used orange peel for years. I never had a problem with the pathologist not approving the control for use on patient stains. Its "naturally occurring", a petri dish would not be naturally occurring in my mind.
Once I verified the orange peel does stain the fungus the same way it does in human tissue by doing a side by side staining the pathologist were fine with it. Actually happy I was able to save some money.
Most controls you get from other source are not human either, usually it some type of animal. Try the orange peel. I did like the idea of using the some type of moldy meat or having your micro department do something for you!!!! I have never tried that, but if it works that would be awesome. Thanks,
Timothy N. Higgins, HT (ASCP), QIHC
> Message: 4
> Date: Mon, 4 Nov 2013 15:07:06 -0500
> From: Patrick Laurie <foreightl <@t> gmail.com>
> Subject: Re: [Histonet] Aspergillus tissue blocks for controls
> To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> <CAKEyg-2KaDkn0oFQURzuPcugQt=Ju8LKK6C8daH+nPjvVsPMJw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
> Hi Jim,
> I have found a couple of ways. First, if you are fortunate to have a micro
> department nearby, they can make a very nice one for you (a Histotip in
> Sakura's Histologic). Another method, which doesn't work the best, can be
> moldy orange peel. And the easiest, you can create your own (moldy
> sausage) or even take some human tissue (fresh lung works the best) and
> leave it with some moldy meat, then you can get a great one.
> Good luck!
> On Mon, Nov 4, 2013 at 10:38 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com> wrote:
> > We are in desperate needs of obtaining GMS controls. In the old days we
> > had all the fungal specimens we needed. Today it is getting hard to find
> > these tissues or controls. Does anyone have any idea where we can get
> > tissue blocks with Aspergillus or does anyone have any other suggestions
> > about GMS controls.
> > James Vickroy BS, HT(ASCP)
> > Surgical and Autopsy Pathology Technical Supervisor
> > Memorial Medical Center
> > 217-788-4046
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