[Histonet] RE: bone marrow aspirations

[Hannen, Valerie]

Diana,

We let the aspirates clot in a petri dish, cover them in formalin to fix  the  clot and then make a block from the fixed clot. Process overnight in the processor and embed the clot in the A.M. This process helps eliminate the aspirate from spreading out in the base mold.

I hope this helps.

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Friday, May 31, 2013 11:01 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] bone marrow aspirations

Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks.  Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen.  Has anyone used histogel or making a button and embed that instead of just filtering the aspirate and putting that is a cassette.

Thanks
Diana
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