[Histonet] fungal stain for frozen sections

robert_schoonhoven robert_schoonhoven <@t> yahoo.com
Thu May 16 14:23:27 CDT 2013


<span style="font-size:10pt;"><p style="margin-top:0;margin-bottom:0;">At what wave length? &nbsp;Eosin &nbsp;is is also fluorescent&nbsp;</p><p style="margin-top:0;margin-bottom:0;">&nbsp;</p><dev3_jjy>Sent from my Boost Mobile phone.</dev3_jjy>

<p>&nbsp;</p><font><p>&nbsp;</p><p>&nbsp;</p>------ Original Message ------<br><b>From:&nbsp;</b>Will Chappells <chapcl <@t> yahoo.com><br><b>Date:&nbsp;</b>5/16/2013 12:18 PM<br><b>To:&nbsp;</b>wrglo5 <@t> aol.com;<br><b>Cc:&nbsp;</b>histonet <@t> lists.utsouthwestern.edu;<br><b>Subject:&nbsp;</b>Re: [Histonet] fungal stain for frozen sections<p>&nbsp;</p><pre>It appears, from a research paper I was reading, that a 1% solution of uvitex 2b added to eosin will cause fungus to fluoresce under uv light. 

I haven't performed it, however it could be as quick as an H&amp;E stain. Looks promising. 

Http://rd.springer.com/article/10.1007%2FBF02889994#page-1

Will Chappell
CHOC children's hospital. 

Sent from my iPhone

On May 16, 2013, at 9:00 AM, wrglo5 <@t> aol.com wrote:

&gt; Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an H&amp;E on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks!
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